用均匀设计法优化瓜尔豆愈伤组织诱导的研究  

Optimization of the Condition of Callus Induction from Guar (Cyamopsis tetragonoloba) by Uniform Design

作　　者：孙爱新[1] 赵飏[1] 权俊萍[2] 辛建华[1] 傅振清[1] 

机构地区：[1]石河子大学农学院园艺系 [2]石河子石河子大学农学院林学系,新疆石河子832003

出　　处：《石河子大学学报（自然科学版）》2004年第5期386-388,共3页Journal of Shihezi University(Natural Science)

基　　金：教育部高等学校骨干教师资助计划项目(GG 0902 10759 3116)

摘　　要：以瓜尔豆子叶和下胚轴为外植体,采用均匀设计法,研究了基本培养基、激素水平、蔗糖浓度和活性炭对愈伤组织形成的影响。通过建立模型表明,瓜尔豆子叶形成愈伤组织的能力高于下胚轴;2,4 D和蔗糖浓度在一定范围内对愈伤组织的形成呈正相关性作用,且外植体、2,4 D和KT浓度三者之间存在很强的交互作用;而活性炭对愈伤组织形成未表现出明显作用。In this paper culture medium,explant,hormone,sucrose and active carbon were studied,which influence the formation of callus in cotyledon and hypocotyl culture of guar.The trail was designed by Uniform Design.The regression model shows that callus induction frequency of cotyledon is higher than it of hypocotyl.The effects of 2,4-dichlorophenoxyacetic acid (2,4-D) and sucrose on callus induction are positive and there is a great interaction between 2,4-D,KT and explant on callus induction from gaur.But the effect of active carbon is not obvious.

关 键 词：瓜尔豆 外植体 均匀设计 愈伤组织诱导 

分 类 号：S515.04[农业科学—作物学]