用gfp基因检测饭豆根瘤菌的侵染途径和定殖动态  

Detecting the colonization and infection pathway of Rhizobiumin rice bean by gfp gene

作　　者：王创[1] 郑慧芬[1] 何绍江[1] 石迪萌[1] 冯娜[1] 张薇[1] 陶雪莹[1] 

机构地区：[1]华中农业大学生命科学技术学院,武汉430070

出　　处：《生物学杂志》2004年第6期14-16,共3页Journal of Biology

基　　金：中国自然科学基金(30070027);华中农业大学SRF项目资助

摘　　要：将绿色荧光蛋白基因转入饭豆根瘤菌JMC1402后,与AMF(ArbuscularMycorrhizalFungi)共同接种饭豆,分别用砂培和土培检测饭豆根瘤菌的侵染途径和定殖动态,并与单接饭豆根瘤菌比较。结果表明,饭豆根瘤菌先吸附在根表,然后从卷曲的根毛侵入。AMF能促进饭豆根瘤菌在根内定殖,抑制饭豆根瘤菌在根表定殖。Rhizobium sp.JMC1402P firstly atabstracted on the surface of ricebean root,then intruded into root from root-hair.Rhizobium sp.JMC1402P was co-inoculated with AMF(Arbuscular Mycorhizal Fungi)in ricebean.The colonization of Rhizobium in ricebeans which were planted both in sand and soil were detected.The result from single-inoculation and co-inoculation treatment showed that AMF could accelerate the Rhizobium's colonization in the root of ricebean and restrict the colonization on the surface of ricebean root.

关 键 词：饭豆根瘤茵 侵染途径 AMF 定殖动态 

分 类 号：Q789[生物学—分子生物学]