紫外分光光度法测定海星皂甙的含量  

Determination of Asterosaponins in Asterias Amurensis and Craspidster Hesperus with Ultraviolet Spectrophotometry

作　　者：文震[1] 朱志鑫[1] 申德妍[1] 党志[1] 

机构地区：[1]华南理工大学环境科学与工程学院,广州510640

出　　处：《分析科学学报》2004年第6期592-594,共3页Journal of Analytical Science

基　　金：教育部高等院校博士点基金(No.20020561026);广东省自然科学基金重点项目(No.036556)

摘　　要：海星皂甙与浓硫酸反应生成α,β 不饱和酮,在277nm处产生特征吸收峰,据此建立了紫外分光光度法测定海星皂甙含量的分析方法。该法测定皂甙的百分吸光系数E1%为149mL·g-1·cm-1,线性范围10～50μg/mL,检出限为0.37μg/mL。方法1cm,λmax用于不同种类海星组织中的皂甙含量测定,简单快速,稳定性好,精密度高,结果令人满意。With the reaction of asterosaponins and sulfuric acid, α,β-unsaturated ketone was formed, and a characteristic absorption peak appeared at 277 nm. Based on the above discovery, a method for determination of asterosaponins with ultraviolet spectrophotometry was presented. The linear range was 10～50 μg/mL, E^(1%)_(1cm,λ_(max)) was 149 mL·g^(-1)·cm^(-1), and the detection limit was (0.37 μg/mL.) This method was used to the analysis of different tissues of two kind of starfish (Asterias amurensis and Craspidster hesperus) with satisfactory results.

关 键 词：紫外分光光度 海星 皂甙 

分 类 号：O657.3[理学—分析化学]