用HPRT基因突变及CBMN研究AT细胞高辐射敏感性  

Study on high radiosensitivity of AT cells by HPRT gene mutation and cytokinesis-block micronucleus method

作　　者：罗加林[1] 曹建平[1] 朱巍[1] 刘芬菊[1] 王明明[1] 周献锋[1] 朱财英[1] 郑斯英[1] 

机构地区：[1]苏州大学放射医学与公共卫生学院放射生物学教研室,苏州215007

出　　处：《辐射研究与辐射工艺学报》2004年第6期365-369,共5页Journal of Radiation Research and Radiation Processing

基　　金：国家自然科学基金(30170288);江苏省 2004 年度研究生创新计划项目(Xm04-67);苏州大学医学发展基金重点项目(EE126032)

摘　　要：研究了毛细血管扩张性共济失调症(Ataxia?telangiectasia,AT)患者皮肤的成纤维细胞系AT5BIVA(AT细胞)的高辐射敏感性。以源于正常人皮肤的成纤维细胞系GM0639(GM细胞)为对照,利用次黄嘌呤磷酸核糖转移酶基因(Hypoxanthinephospho?ribosyltransferase,hprt)位点突变分析技术及胞质分裂阻滞微核法(Cytokinesis?Blockmicronucleusmethod,CBMN),在AT细胞和GM细胞经Coγ射线0、1、2、3、4Gy60照射后,观察比较AT细胞和GM细胞之间hprt基因位点突变频率(hprtMF)、微核率(MNF)及微核细胞率(MNCF)的差异,并分别进行曲线拟合。在各剂量点,AT细胞hprt基因突变频率、微核率及微核细胞率均明显高于GM细胞,其差别具有显著性统计意义(p<0.01);AT和GM细胞hprt基因突变频率、微核率及微核细胞率均与照射剂量呈正相关,可拟合成剂量效应直线方程y=a+bx。结果表明,毛细血管扩张性共济失调症患者AT细胞辐射敏感性显著高于GM细胞,具有高辐射敏感性。The high radiosensitivity of fibroblast cells (AT5BIVA cells) from the skin of the Ataxia- telangiectasia (A-T) patients were studied. Using Hypoxanthine phospho-ribosyl transferase gene mutation analysis and Cytokinesis-Block micronucleus method, the hprt mutation frequence(hprtMF), the micronucleus frequencies (MNF)and micronucleus cell frequencies (MNCF)of AT and GM cells irradiated with 0, 1, 2, 3 and 4Gy of Co γ-rays 60 were observed. The difference of hprtMF, MNF and MNCF between AT and GM cells were analyzed, and dose-response curves of hprtMF, MNF and MNCF in AT and GM cells were also established. After exposure to 0, 1, 2, 3 and 4Gy of 60Co γ-rays, hprtMF, MNF and MNCF were significantly higher in AT cells than those in GM cells (p<0.01). In the two cell types, hprtMF MNF and MNCF had a positive correlation with dose, and their linear regression equations were y=a+bx. These results show that the radiosensitivity of AT cells is significantly higher than that of GM cells and AT cells are extremely sensitive to radiation.

关 键 词：毛细血管扩张性共济失调症 高辐射敏感性 次黄嘌呤磷酸核糖转移酶基因 胞质分裂阻滞微核法 

分 类 号：X591[环境科学与工程—环境工程] R114[医药卫生—卫生毒理学]