大鼠全脑照射后早期海马组织内炎性细胞因子的变化特点  

The expression changes of inflammatory cytokines in the hippocampus following whole-brain irradiation in rats

作　　者：余德[1] 田野[1] 丁维军[1] 朱雅群[1] 刘春风[1] 

机构地区：[1]苏州大学附属第二医院,苏州215004

出　　处：《辐射研究与辐射工艺学报》2004年第6期370-374,共5页Journal of Radiation Research and Radiation Processing

基　　金：国家自然科学基金(30170287)资助

摘　　要：探讨大脑受辐射后炎性细胞因子在脑组织内的变化特征。对被单次全脑2、15、30Gy照射后1h至1w的(Sprague?Dawley,SD)大鼠,用酶联免疫吸附法(ELISA)和核糖核酸(RNA)逆转录聚合酶链反应(RT?PCR)分别检测大脑海马组织中IL?1β、IL?6和TNF?α含量和其基因转录产物水平。结果表明,15Gy和30Gy照射后12h内,海马中IL?1β、TNF?α含量与mRNA相对量有显著升高;IL?6含量虽无明显变化,但其mRNA亦有明显升高,这些改变在照射后1d时可恢复至正常水平。受较高剂量全脑辐照后,1d内脑内主要炎性细胞因子的表达量有迅速地增加。To investigate the change pattern of some inflammatory cytokines in brain tissue at the acute phase after brain irradiated. The whole brain of SD rats was irradiated by the single dose of 2, 15, or 30Gy of 4 MeV elec- tron beam. The enzyme?linked immunosorbent assay (ELISA) was used for the measurement of IL?1 β, IL?6, and TNF?α content in hippocampus tissue of rats at 1h, 6h, 12h, 1d, 2 and 1week post?irradiation. The mRNA of IL?1 β, IL?6, and TNF?α were detected by reverse?transcription polymerase chain reaction (RT?PCR) in the same experi- mental groups. It was analyzed about the influence of dosage and post?irradiation duration with the cytokines expres- sion. Compared with both the normal control and the anaesthetized with chloral hydrate but sham?irradiation groups, there were no difference about the three inflammatory cytokines expression in rats with 2Gy irradiated. At 6h after ir- radiation with 15Gy, 6 and 12h with 30Gy groups, the content of IL?1 β and TNF?α in hippocampus tissue were sig- nificantly increased, and were returned to normal level after 12 to 24h. The same change tendency of their mRNA re- lational level was observed in 15 and 30Gy groups, but it happened earlier in 1h after exposure. Although the content of IL?6 in hippocampus kept stable in all the groups, its mRNA level raised obviously in 12h group. After 15—30Gy whole?brain irradiation, the expression of some inflammatory cytokines increased abruptly in the hippocampus of SD rat within 1 day, but the interplay between inflammatory cytokines changes and the pathogenesis of radiation injury was incompletely understood at present.

关 键 词：大脑 电离辐射 炎性细胞因子 海马 大鼠 

分 类 号：R818.74[医药卫生—放射医学]