基于功能性CuS纳米粒子共振光散射法测定蛋白质(英文)  

Resonance Light Scattering Technique for the Determination of Proteins with Functionalized Nano-CuS

作　　者：张明翠[1] 张德兴[1] 汪乐余[1] 夏婷婷[1] 董玲[1] 王伦[1] 

机构地区：[1]安徽师范大学化学与材料科学学院,安徽芜湖241000

出　　处：《安徽师范大学学报（自然科学版）》2004年第3期295-298,共4页Journal of Anhui Normal University(Natural Science)

基　　金：NationalNatureScienceFoundationofChina( 2 0 3 75 0 0 1) ;SpecialFoundationofAnhuiNormalUniversity ( 2 0 0 4xzx0 2 ) ;NaturalScienceFoundationforYoungTeacherofAnhuiProvinceEducationCommission ( 2 0 0 4jq12 1)

摘　　要：基于蛋白质的加入使功能性CuS纳米粒子的共振光散射明显增强 ,建立了定量测定蛋白质的新方法 .最佳实验条件下 ,对人血清球蛋白的线性范围为 0 .1- 7.0 μgml- 1,检出限为 13ngml- 1,对人血清白蛋白的线性范围为 0 .2 5 - 11.0 μgml- 1,检出限为 2 6ngml- 1.该方法成功用于人血清样品的测定 ,方法简单、灵敏、稳定。Based on the strong enhancement effect of proteins on resonance light scattering of functionalized nano-CuS at 383 nm, a new direct quantitative determination method for proteins in blood serum samples is established. Under optimal conditions, the enhanced RLS intensity is in proportion to the globulin concentration in the range 0.1-7.0 (g ml^(-1). The limit of detection is 13 ng ml^(-1). And the dynamic range for human serum albumin is 0.25-11.0 (g ml^(-1) with detection limit of 26 ng ml^(-1). Human blood serum samples were determined satisfactorily. This method is characterized by high sensitivity, simplicity, good selectivity and stability of the chemical system.

关 键 词：人血清白蛋白 蛋白质 功能性 球蛋白 定量测定 灵敏 共振光散射法 检出限 纳米粒子 线性范围 

分 类 号：R446[医药卫生—诊断学] O657[医药卫生—临床医学]