基于疏水性小波分析的膜蛋白结构预测  

Prediction of Transmembrane Protein Structure Based on Hydrophobicity Wavelet Analysis

作　　者：邱建丁[1] 荣联清[2] 梁汝萍[1] 谭学才[1] 邹小勇[1] 莫金垣[1] 

机构地区：[1]中山大学化学与化学工程学院,广东广州510275 [2]萍乡高等专科学校化工系,江西萍乡337055

出　　处：《分析测试学报》2004年第6期1-4,8,共5页Journal of Instrumental Analysis

基　　金：国家自然科学基金资助项目 (29975033) ;广东省自然科学基金资助项目 (001237;031577)

摘　　要：膜蛋白在细胞膜上具有重要的生理功能 ,大部分膜蛋白在药物设计、转运蛋白和免疫识别等方面起着关键的作用 ,从分子水平上预测这类蛋白质的结构具有非常重要的意义。本文提出一种基于氨基酸疏水性小波变换技术预测膜蛋白跨膜区段数目和位置的新方法。以代码为upkb_bovin的膜蛋白为例 ,对跨膜螺旋区数目和位置的预测分析进行了描述。从膜蛋白数据库中随机抽取36个蛋白质 (含跨膜螺旋区232)作为测试集检验小波分析的预测方法 ,其中226个跨膜螺旋区能被准确预测 ,准确率为96.8%。结果表明 。Transmembrane(TM)proteins possess important physiological function.A large portion of them play crucial roles in drug receptors,transporters,and immunological recognition.The prediction of the strucˉtures of these TMproteins at molecular level is thus very important and meaningful.A newmethod based on the combination of wavelettransform(WT)and the hydrophobicity of amino acids for the prediction of the numˉber and location of the transmembrane helices in membrane proteins was developed.To investigate the feasibilˉity of this method,the TMprotein,coded as upkb-bovin was chosen as an example.Thirty six proteins conˉsisting of232transmembrane helices were randomly selected from the TM protein databank.It was found that96.8%of the transmembrane helics were accurately predicted.

关 键 词：疏水性 小波变换 膜蛋白 结构 预测 跨膜螺旋区 

分 类 号：Q51[生物学—生物化学] Q73