聚结晶紫薄膜修饰电极测定尿酸  

Use of Poly(Crystal Violet)Film Modified Electrode to Measure Uric Acid

作　　者：周谷珍[1] 孙元喜[1] 

机构地区：[1]湖南文理学院化学化工系,湖南常德415000

出　　处：《分析测试学报》2004年第6期18-21,共4页Journal of Instrumental Analysis

基　　金：湖南省教育厅资助课题(02C120)

摘　　要：利用循环伏安法制备了聚结晶紫薄膜修饰电极(PCVE) ,详细研究了该修饰电极对生物分子尿酸 (UA)的电催化作用。结果表明 ,PCVE对UA具有较强的电催化作用 ,并且对抗坏血酸(AA)具有较强的抗干扰作用 ,允许高达1000倍以上AA存在而不干扰痕量UA的测定。将PCVE结合差分脉冲伏安(DPV)技术 ,对UA的检测线性范围为5.0×10 -7～5.0×10 -5mol/L,检出限达7.5×10 -8mol/L。将该法用于尿液中尿酸的测定 。A poly(crystal violet)film modified electrode(PCVE)was prepared by cyclic voltammetry,and the electrocatalytic functionality of PCVE for a biological molecule,uric acid(UA),was studied.The results showed that PCVE has strong electrocatalytic functionality for UA,and strong anti-jamming impact for ascorˉbic acid(AA).Determination of UA was possible in the presence of AA concentration up to1000times that of UA.When PCVE was combined with difference pulse voltammetry(DPV),the linear detection range of UA concentrations was from5.0×10 -7 to5.0×10 -5 mol/L with a method detection limit of7.5×10 -8 mol/L.Use of this method for measurement of UA in urine obtained satisfactory results.

关 键 词：聚结晶紫薄膜修饰电极 尿酸 循环伏安法 电催化 尿液 含量测定 

分 类 号：R446.12[医药卫生—诊断学]