Electrochemical Determination of Alkaline Phosphatase in Human Serum by Differential Pulse Voltammetry  

作　　者：SUNWei JIAOKui WANGHai-yu LULu-de 

机构地区：[1]CollegeofChemicalEngineering,NanjingUniversityofScienceandTechnology,Nanjing210094,P.R.China [2]CollegeofChemistryandMolecularEngineering,QingdaoUniversityofScienceandTechnology,Qingdao266042,P.R.China

出　　处：《Chemical Research in Chinese Universities》2004年第6期690-693,共4页高等学校化学研究（英文版）

基　　金：the National Natural Science Foundation of China(No.2 0 0 75 0 13) and China Postdoctoral Science Foun-dation(No.2 0 0 30 33492 )

摘　　要：Differential pulse voltammetry(DPV) was applied to the determination of alkaline phosphatase(ALP) activity in human serum with phenyl phosphate as the substrate. Phenyl phosphate can enzymatically be hydrolyzed to produce phenol which is quantified by DPV at +660 mV(vs.Ag/AgCl) in the concentration range of 2.0_100 μmol/L. The standard curve for ALP is linear over the range from 0.06 to 1000 U/L with a relative standard deviation of 3.0%. The conditions for the enzymatic reaction and voltammetric detection were optimized and the kinetic constants were also examined.The human serum samples were tested by this method and the results were in good agreement with those obtained by the routine p-nitrophenyl phosphate spectrophotometric method.Differential pulse voltammetry(DPV) was applied to the determination of alkaline phosphatase(ALP) activity in human serum with phenyl phosphate as the substrate. Phenyl phosphate can enzymatically be hydrolyzed to produce phenol which is quantified by DPV at +660 mV(vs.Ag/AgCl) in the concentration range of 2.0_100 μmol/L. The standard curve for ALP is linear over the range from 0.06 to 1000 U/L with a relative standard deviation of 3.0%. The conditions for the enzymatic reaction and voltammetric detection were optimized and the kinetic constants were also examined.The human serum samples were tested by this method and the results were in good agreement with those obtained by the routine p-nitrophenyl phosphate spectrophotometric method.

关 键 词：Alkaline phosphatase Phenyl phosphate Enzymatic analysis Differential pulse voltammetry Human serum sample 

分 类 号：O629.8[理学—有机化学] Q556.1[理学—化学]