细基江蓠及其繁枝变种的RAPD和ITS分析  

RAPD detection and ITS sequences analysis of Gracilaria tenuistipitata and G. tenuistipitata var. liui

作　　者：李文红[1] 胡自民[1] 覃志彪[2] 郭亚芬[2] 王如才[1] 段德麟[3] 

机构地区：[1]中国海洋大学水产学院,山东青岛266003 [2]广西大学水产系,广西南宁530005 [3]中国科学院海洋研究所,山东青岛266071

出　　处：《海洋学报》2004年第6期89-95,共7页

基　　金：国家"863"高技术发展计划资助项目(2001AA621090);广西科学基金资助项目(0236011);青岛市科技局资助项目(03-2-JSH-02).

摘　　要：对细基江蓠及其繁枝变种各12个个体进行随机扩增多态性(RAPD)DNA分析,对比多态位点比例、平均杂合度以及遗传距离,并构建UPGMA聚类图;通过PCR扩增出核糖体RNA基因(rDNA)转录间隔区(ITS),纯化后直接测序,利用生物信息学方法进行序列分析和核苷酸变异研究.比较分析结果表明,细基江蓠的杂合度和多态位点比例均高于细基江蓠繁枝变种,其遗传多样性相对较丰富;实验中8条随机引物扩增出特异的RAPD带,可用做细基江蓠及其繁枝变种的分子鉴定标记;ITS序列在这两种海藻中变异极小;RAPD和ITS聚类分析研究结果表明,细基江蓠及其繁枝变种均聚集为一枝,与同属不同种的龙须菜和真江蓠分开,提示细基江蓠和及其繁枝变种为同一个种,从而在DNA水平上支持了传统形态分类的观点.RAPD detection and ITS1, 5.8 s rDNA sequencing analysis were conducted to study the genetic variation and phylogenesis of Gracilaria tenuistipitata and G. tenuistipitata var. liui. The proportion of polymorphic, average heterozygosity and genetic distance were compared, a cluster tree was constructed by UPGMA from TFPGA. The results show that the genetic diversity of G. tenuistipitata is higher than G. tenuistipiitata var. liui. Eight RAPD primers were selected through the screening from the 300 primers, and its yielded specific markers for verification of G. tenuistipitata and G. tenuistipitata var. liui. Only one base difference was found from the ITS1 and 5.8s rDNA sequences analysis between G. tenuistipitata and G. tenuistipitata var. liui. it is indicated that RAPD method can be used for Gracilaria germplasm verification and genetic analysis, and it is confirmed that G. tenuistipitata and G. tenuistipitata var. liui belong to the same species, which meant that G. tenuistipitata var. liui is the morphological variant of G. tenuistipitata.

关 键 词：细基江蓠 细基江蓠繁枝变种 RAPD ITS 分类 鉴定 

分 类 号：Q949.2[生物学—植物学]