表达PRRSVE基因重组质粒在小鼠诱导的体液免疫应答  

Humoral immune response evicited by the recombinant plasmid expressing E gene of PRRSV in mice

作　　者：高云[1] 杨汉春[1] 刘平黄[1] 陈声[1] 郭鑫[1] 韩军[1] 黄芳芳[1] 

机构地区：[1]中国农业大学农业部预防兽医学重点开放实验室,北京海淀100094

出　　处：《中国兽医杂志》2004年第11期6-8,共3页Chinese Journal of Veterinary Medicine

基　　金：教育部霍英东青年教师基金资助 ;国家十五攻关子课题 ( 2 0 0 2 BA5 14 A-16-0 5 )

摘　　要：利用 PCR技术对猪繁殖与呼吸综合征病毒 (PRRSV) BJ- 4株的 E基因进行修饰和改造 ,在 E基因上游加入 Kozak序列 ,扩增并克隆 E基因。将 E基因 c DNA亚克隆至真核表达载体 pc DNA3.1(+)中 ,构建了真核重组表达质粒 pc DNA- E。用pc DNA- E免疫小鼠 ,经免疫荧光抗体试验检测结果表明 ,重组质粒 pc DNA- E经 3次免疫后 ,所有小鼠血清抗体均为阳性 ,说明pc DNA- E在小鼠体内可诱导特异性的体液免疫应答反应。By PCR, the ORF5 (E gene) of PRRS virus BJ-4 strain was modified and amplified, and sub-cloned into expression vector pcDNA3.1(+). The recombinant expression plasmid pcDNA-E was inoculated into mice. The antibodies specific for E protein in serum were confirmed by IFA. The antibody could be detected in all the mice after the third inoculation.The results showed that the recombinant plasmid pcDNA-E induced specific humoral immune responses in mice.

关 键 词：猪繁殖与呼吸综合征病毒 E基因 重组质粒 体液免疫应答 表达 

分 类 号：S852.65[农业科学—基础兽医学] Q78[农业科学—兽医学]