细胞表面糖链末端唾液酸和岩藻糖与某些细胞生物学行为的关系  

RELATIONSHIP BETWEEN TERMINAL SIALYL AND FUCOSYL RESIDUES OF GLYCANS ON CELL SURFACE AND CELL BIOLOGICAL BEHAVIORS

作　　者：张英[1] 张夏英[1] 刘飞[1] 齐慧玲[1] 陈惠黎[1] 

机构地区：[1]卫生部糖复合物重点实验室

出　　处：《实验生物学报》2002年第4期271-277,共7页Acta Biologiae Experimentalis Sinica

基　　金：国家自然科学基金重点项目(No.396300800)资助

摘　　要：用神经氨酸酶和α-L-岩藻糖苷酶分别切除人肝癌细胞株7721细胞表面糖链中的末端唾液酸(SA)和岩藻糖(Fuc)残基来研究表面聚糖结构和某些细胞生物学行为之间的关系。选择细胞对纤连蛋白(Fn)、层黏蛋白(Ln)和人脐静脉内皮细胞(HUVEC)的黏附能力、细胞趋化性迁移以及趋化性侵袭作为细胞行为的指标。结果表明:表面糖链末端SA对细胞黏附至Fn并不必需,对细胞黏附至Ln和细胞的趋化性侵袭却至为重要,而对细胞黏附至HUVEC以及趋化性迁移则为关键性残基。与SA相比,Fuc可能参与细胞Fn、Ln和HUVEC的黏附,但对趋化性迁移以及趋化性侵袭并不重要。细胞对HUVEC的黏附以及趋化性迁移和侵袭可被唾液酸化Lewis X(SLe^x)的单抗抑制,但不被未唾液酸化的Lewis X(Le^x)单抗抑制,这一结果支持SA在上述三种细胞过程中较Fuc残基重要。The relationship between the structures of glycans on the surface of H7721 cells, a human hepa-tocarcinoma cell line, and the cell behaviors was studied by using neuraminidase and α-L-fucosidase to remove the terminal sialyl or fucosyl residues of surface glycans respectively. The cell adhesion to fibronectin (Fn), laminin (Ln) and human umbilical vein epithelial cell (HUVEC) , as well as cell chemotactic migration and invasion were selected as the parameters of the cell behaviors. It was found that sialyl residue was not essential for the cell adhesion to Fn, but was important in the cell adhesion to Ln and chemotactic cell invasion, and very crucial in the cell adhesion to HUVEC and chemotactic migration. In contrast, fucosyl residue was probably participate in cell adhesion to Fn, Ln and HUVEC, but not important in chemotactic migration and invasion. The cell adhesion to HUVEC, chemotactic migration and invasion were inhibited by the monoclonal antibody of sialyl Lewis X (SLex) , but not by the antibody of non-sialyl Lewis X (Lex). This result supports the finding that sialyl residue is more important than fucosyl residue in the contribution to the above-mentioned three cell processes.

关 键 词：侵袭 HUVEC 细胞黏附 细胞生物学行为 细胞表面 糖链 SA 趋化性 残基 单抗 

分 类 号：Q26[生物学—细胞生物学]