不同激活方法对猪体外成熟卵母细胞孤雌发育的影响  

Effect of Different Activation Methods on the Development of Porcine Oocytes Matured in vitro

作　　者：刘国世[1] 曾申明[1] 吴中红[1] 邢凤英[2] 田见晖[1] 林平[1] 姜午旗[1] 刘敬浩[1] 朱士恩[1] 张忠诚[1] 

机构地区：[1]中国农业大学动物科技学院,北京100094 [2]上海第二医科大学发育生物学重点实验室,上海200025

出　　处：《畜牧兽医学报》2004年第6期615-620,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：中国博士后科学基金(2001年)资助

摘　　要：研究了猪体外成熟卵母细胞的电激活、离子霉素激活和乙醇激活的方法。3种不同激活方法筛选试验表明,猪卵母细胞电激活最佳参数为电场强度130V/min,脉冲时程80μs的1次脉冲激活,即130V/mm-80μs-1次,其囊胚(发育)率为18.92%±8.48%(P>0.05);离子霉素激活的最佳条件为15μmol/L、激活时间40min,其囊胚率为21.27%±8.54%(P>0.05);乙醇激活最佳参数以9%乙醇激活处理3min,囊胚率为13.33%±7.64%。进一步对比试验表明,电激活和离子霉素激活处理的囊胚率和囊胚细胞数无显著差异(P>0.05),电激活的卵裂率明显高于乙醇激活(P<0.05),而囊胚率和细胞数差异不显著(P>0.05)。Electrical, ionomycin and ethanol activation of porcine oocytes matured in vitro were performed in this study to establish an effective activation protocol for parthenogenetic porcine oocytes. Oocytes were parthenogenetically activated by one of three stimuli: (1) optimal electric (electrical field strength and the pulse duration 130 V/mm-80 μs with blastocyst development rate of 18.92%±8.48%), (2) optimal ionomycin (15 μmol/L ionomycin for 40 min with blastocyst rate of 21.27%±8.54%), (3) optimal ethanol (9% ethanol for 3 min followed, resulting in blastocyst rate of 13.33%±7.64%). It was proved that in the same circumstance no obvious difference existed in the blastocyst rate (32.70%±13.55% vs. (26.58%±)13.83%) and cell number of blastocyst between electrical activation (130 V/mm-80μs-1)and inomycin activation(15 μmol/L,40 min). Moreover, electrical activation achieved higher(P<0.05) rate of post-activation cleavage than ethanol activation; the blastocyst rate(13.21%±6.05% vs. 5.00%±(1.80%)) and cell number of blastocyst showed no difference(P>0.05). Therefore, among the three kinds of activation methods, electrical activation was the best.

关 键 词：囊胚率 猪体 激活方法 体外成熟 卵母细胞 孤雌发育 筛选试验 霉素 细胞数 乙醇 

分 类 号：S823[农业科学—畜牧学] R737.25[农业科学—畜牧兽医]