体外试验中不同粗精比对山羊瘤胃细菌氨态氮利用效率的影响  

Effects of Different Roughage and Concentrate Ratios on Ruminal Bacterial Utilization of Ammonia-N in Goat in vitro

作　　者：赵国琦[1] 丁健[1] 孙龙生[1] 张艳云[1] 刘大林[1] 贾亚红[1] 陈小连[1] 

机构地区：[1]扬州大学动物科学与技术学院,扬州225009

出　　处：《畜牧兽医学报》2004年第6期640-645,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：江苏省自然科学基金(BK99094);国家自然科学基金(30270962)资助

摘　　要：利用体外发酵法研究了4种粗精比(10∶0、8∶2、6∶4和4∶6)底物对山羊瘤胃细菌产量、15NH3-N利用效率的影响。结果表明:混合培养液的pH值与氨态氮浓度在8h内急剧下降,而后至24h呈缓慢下降;经24h培养的细菌产量在8∶2、6∶4和4∶6组中差异不显著(P>0.05),但都极显著地高于10∶0组(P<0.01);细菌体15N丰度以8∶2及4∶6组较高,显著高于10∶0和6∶4组(P<0.05);细菌体15N富集量以8∶2组最高,底物547.43μg15N中有120.44μg被细菌体富集,显著高于10∶0组(P<0.05),但6∶4、4∶6组与10∶0组间差异均不显著(P>0.05)。细菌赖氨酸的相对百分组成随精饲料比例的增加而显著增加,胱氨酸在粗饲料组(10∶0)显著高于其它组,但各精饲料添加组间差异不显著,组氨酸以8∶2组为最高,显著高于10∶0组,但与其它精饲料添加组间差异不显著(P>0.05)。An in vitro system was used to investigate the influence of different roughage and concentrate ratio substrates on utilization of ammonia-N by mixed ruminal bacteria in vitro. The treatments were four roughage and concentrate ratio substrates (10∶0, 8∶2, 6∶4 and 4∶6). The pH and ammonia-N concentration rapidly decreased in first 8 h incubation, then the speed of decrease became slow in later incubation. At the end-point of incubation (24 h), bacteria isolated from 8∶2, 6∶4 and 4∶6 groups had much higher biomass compared with that in 10∶0 group (P<0.01). ()^(15)N Atom % abundance of bacteria was much higher in 8∶2 and 4∶6 groups than that in 10∶0 and 6∶4 groups (P<0.05). The highest level of ()^(15)N-enrichment was observed in the 8∶2 group, of the 547.43 μg ()^(15)N provided in the medium, 120.44 μg ()^(15)N was utilized over the incubation period, much higher than that in 10∶0 group (P<0.05), but no differences compared with the 6∶4 and 4∶6 groups (P>0.05). The Lys content of bacteria increased with the level of concentrate feedstuff inclusion in the substrate, the content of (Cys)_2 was higher in 10∶0 group compared with other three groups, His content in 8∶2 group was at the highest level, no differences compared with the 6∶4 and 4∶6 groups, but much higher than that in 10∶0 group.

关 键 词：^15N 精饲料 瘤胃 山羊 氨态氮 氮利用效率 细菌 体外试验 影响 比对 

分 类 号：S823[农业科学—畜牧学] S816[农业科学—畜牧兽医]