抗猪大肠杆菌高免卵黄的研制及对实验感染病例的疗效观察  

Preparation and effect of egg yolk antibody with strong immunity against experimental Echerichia coli K88 strain infection in neonatal piglets

作　　者：党如意[1] 杨增岐[1] 孙世铎 杜恩岐[1] 张淑霞[1] 张改智 

机构地区：[1]西北农林科技大学动物科技学院，陕西杨凌712100 [2]不详 [3]杨陵区畜牧兽医工作站，陕西杨凌712100

出　　处：《西北农林科技大学学报（自然科学版）》2004年第B11期50-53,共4页Journal of Northwest A&F University(Natural Science Edition)

摘　　要：本试验主要选择了引起猪黄白痢的常见致病菌株E．coli K88，通过对其在专用培养基上增菌培养，制成油佐剂灭活苗免疫产蛋母鸡，运用微量凝集试验测母鸡抗体水平，待抗体水平达8log2以上时，收取鸡蛋制成高免卵黄。选择6窝56头刚出生的未吮乳仔猪(母猪未免疫)，第1，2窝16头用E．coli K88标准菌株500亿／头口服人工感染，第3，4窝18头口服E．coli K88标准菌株250亿／头，第5窝10头作为抗生素治疗对照组，第6窝12头为空白对照组，观察记录发病情况，待明显发病后，对第1～4窝仔猪用自制抗E．coli K88高免卵黄口服治疗。试验结果表明，口服抗E．coli K88菌株高免卵黄抗体对人工感染仔猪黄痢与白痢的治愈率分别为93．75％和88．89％，而应用抗生素庆大霉素的治愈率仅为50％。This experiment selected laying hens innoculated with inactivated oil emulsion vaccine, which was made from Echerichia coli (E. coli) K88 strain. The serum antibody titer of the hens against E. colt K88 strain was tested by micro-agglutination test. The egg yolk antibody liquids were made from the eggs which were collected when the antibody titer arrived at 8 log2 or more. 56 piglels in 6 litters were infecled with E. coli K88 strain and then were oral applied with egg yolk antibody liquids for treatment when the typical clinic symptoms of the piglets were obvious. The results indicated that the cure rate of egg yolk antibody for treatment of yellow scour and white scour of newborn piglets was 93. 75% and 88. 89%,respectively. While the cure rate of Gentamycin was only 50%. Reoccurrence was not observed.

关 键 词：高免卵黄抗体 人工感染 实验感染 油佐剂灭活苗 产蛋母鸡 乳仔猪 仔猪黄痢 口服 E.coli 抗E 

分 类 号：S858[农业科学—临床兽医学] S831[农业科学—兽医学]