蔗糖梯度离心法纯化Vero细胞乙脑疫苗的纯度分析及与层析法的比较  

The analysis of purity of Japanese encephalitis vaccine purified on sucrose density gradient centrifugation and the comparison with sephacryl s 1000 agrose chromatography

作　　者：杨立清[1] 张晋[1] 卢立新[2] 李红[1] 石男[1] 

机构地区：[1]北京生物制品研究所,北京100024 [2]北京市西城区疾病预防控制中心,北京100029

出　　处：《微生物学免疫学进展》2004年第4期11-13,共3页Progress In Microbiology and Immunology

摘　　要：通过对蔗糖梯度离心法纯化Vero细胞乙脑疫苗的纯化工艺进行分析,发现现行工艺中收取的病毒组分中,不同蔗糖浓度中病毒的纯度是不同的,而呈峰型且与病毒效力峰及蛋白浓度峰不重合。实验结果对今后工艺的改进具有指导意义。另外应用Sephacry1s1000凝胶层析法对乙脑病毒进行了纯化研究和分析,发现蔗糖梯度离心法和层析法纯化的病毒的纯度及回收率分别为291.46u/mg、96.01%和309.41u/mg65.08%,Sephacry1s1000凝胶层析法同样可以获得较高的纯度和收率。In the present study,two different methods (sucrose density gradient centrifugation and sephacryl s 1000 agrose chromatography) were compared in purification of Japanese encephalitis virus growing in Vero cells. The purity and recovery of the method of sucrose density gradient centrifugation were 291.46 u/mg and 96.01% ,and the method of sephactyl s 1000 agrose chromatography were 309.41 u/mg and 65.08% respectively.Different recovered fractions were different in purity

关 键 词：乙脑疫苗 纯化 层析法 梯度离心 

分 类 号：R392[医药卫生—免疫学]