伤寒Vi-rEPA结合疫苗在小鼠中的免疫原性研究  

Immunogenicity study of typhoid Vi-rEPA conjugate vaccine in mice

作　　者：蒲江[1] 杜琳[1] 谭小梅[1] 侯亚莉[1] 蒋奕[1] 金学敏[1] 谢贵林[1] 

机构地区：[1]兰州生物制品研究所,兰州730046

出　　处：《微生物学免疫学进展》2004年第4期14-17,共4页Progress In Microbiology and Immunology

摘　　要：以伤寒Vi多糖疫苗及结合物含量分别为20%、40%、60%的伤寒VirEPA结合疫苗经皮下免疫小鼠后眼眶采血分离血清,用ELISA法测血清抗体浓度。血清抗体分析表明,伤寒Vi多糖疫苗和伤寒VirEPA结合疫苗均有良好的免疫原性,但伤寒VirEPA结合疫苗的免疫原性优于伤寒Vi多糖疫苗;伤寒Vi多糖疫苗无加强免疫应答效应,而伤寒VirEPA结合疫苗有加强免疫应答效应;不同高分子结合物含量的伤寒VirEPA结合疫苗免疫小鼠,其结合物含量为40%、60%的伤寒VirEPA结合疫苗比结合物含量为20%的结合疫苗具有更好的免疫原性,而前二者的免疫原性无显著性差异。In this study, mouse model was used to verify the immunogenicity of typhoid Vi rEPA conjugate vaccine with different composition of high molecule weight conjugate(HMWC). Three kinds of composition of conjugate vaccines were used. BALB/c mice were immunized subcutaneously 3 times with conjugate vaccines, and blood samples were bled 1 week after each injection to determine the specific anti Vi IgG level with ELISA. The results showed the vaccines contained 40% and 60% of HMWC could elicit higher antibody level than that of 20% (P<0.001). It also showed that booster response could be revealed in conjugate vaccines after boost immunizing for 2 and 3 times while the Vi polysaccharide vaccine could not be.

关 键 词：伤寒Vi-rEPA结合疫苗 高分子结合物 免疫原性 

分 类 号：R392[医药卫生—免疫学]