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出 处:《实验生物学报》2004年第6期456-460,共5页Acta Biologiae Experimentalis Sinica
基 金:国家自然科学基金重点项目(39930100)。
摘 要:本实验对黑腹果蝇种组(melanogaster species group)中8个种亚组33个样品两个线粒体基因ND4和ND4L进行了测序,并分析了ND4基因的序列差异和碱基替换特点,发现近缘物种中存在很明显的转换倾向,而在远缘物种中由于重复替换导致转换数处于饱和状态,我们的实验数据证实了线粒体基因较核基因有较快的进化速度。最后根据D.melanogaster与D.yakuba的遗传距离推算了8个种亚组的分化时间,ananassae种亚组最先分化,然后依次是montium,melanogaster,fic-sphila,eugracilis,elegans,suzukii和takahashii最后分化。The sequences of the mitochondrial ND4-ND4L were determined for all the 33 ex- tant taxaes of the Drosophila melanogaster species group,so evolutionary traits of ND4 gene se- quences were analyzed.We found a strong transition bias in closed related species and saturation of transitional substitutions in distant related species.Moreover,we calculated the diverge time of eight subgroup according to the Tamura-Nei distance between D.melanogaster and D.yakuba.Our result suggested that ananassae subgroup branched off firstly,and montiurrt,melanogaster,ficsphila, eugracilis,elegans,suzukii-takahashii branched off sequentially.
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