成纤维细胞条件培养基对雪旺细胞生长和增殖的影响  

Effects of conditioned mediums of fibroblast on the growth and proliferation of Schwann cells

作　　者：杨俊[1] 张黎声[2] 武雷[1] 邱小忠[1] 余磊[1] 秦建强[1] 

机构地区：[1]解放军第一军医大学临床解剖学研究所,广东省组织构建与检测重点实验室,广东省广州市510515 [2]上海中医药大学解剖学教研室,上海市201400

出　　处：《中国临床康复》2004年第35期7956-7957,i002,共3页Chinese Journal of Clinical Rehabilitation

基　　金：国家自然科学基金(39970833A003030306)资助项目;广东省科技计划专项(2003A3020101)资助项目~~

摘　　要：目的:观察成纤维细胞条件培养基对雪旺细胞增殖分化的影响,探讨两者之间的协同作用,为组织工程化神经桥接体的构建奠定实践基础。方法:取20只出生四五天的SD乳鼠的臂丛和坐骨神经,运用双酶结合单酶消化法培养雪旺细胞和成纤维细胞,纯化和培养雪旺细胞并制备两种条件培养基:A组:成纤维细胞+DMEM/F12;B组:成纤维细胞+雪旺细胞+DMEM/F12;C组:DMEM/F12(对照组)。将A,B,C3组分别加入种植有纯化雪旺细胞的96孔板中,MTT检测雪旺细胞增殖情况,S-100蛋白免疫组化染色鉴定雪旺细胞。结果:B组对雪旺细胞的增殖和分化有明显的促进作用;A组次之;C组最差(P<0.001)。结论:成纤维细胞条件培养基可促进雪旺细胞的增殖和分化。AIM:To observe the effects of fibroblast conditioned mediums on proliferation and differentiation of Schwann cells and explore the synergistic action between them in order to lay the practical foundation for the construction of tissue-engineered bridging substances. METHODS:The sciatic nerve and brachial plexus of twenty 4-5 days old SD rats were taken, and Schwann cells and fibroblasts were cultured with double enzyme digestion and single enzyme digestion method.Schwann cells were purified and cultured and two kinds of conditioned mediums were prepared:group A:fibroblasts+DMEM/F12;group B:fibroblasts+Schwann cells+DMEM/F12;group C: DMEM/F12(control group).Three groups were respectively added into the 96-pore plate of implanting purified Schwann cells.The condition of Schwann cells' proliferation was detected by MTT.Schwann cells were evaluated by S-100 protein immunohistochemical staining. RESULTS:The best promotive effect of proliferation and differentiation acting on Schwann cells was deduced by group B.Group A was next and the worst one was group C(P< 0.001). CONCLUSION:Conditioned mediums of fibroblasts can promote proliferation and differentiasion of Schwann cells.

关 键 词：生物医学工程 成纤维细胞 许旺细胞 

分 类 号：R318[医药卫生—生物医学工程]