人胚胎嗅鞘细胞植入鼠半横断脊髓对脊髓轴突再生的影响  

Effect of implantation of human embryonic olfactory ensheathing cells on the regeneration of transected spinal cord

作　　者：沈慧勇[1] 王学文[1] 唐勇[1] 吴燕峰[2] 杨睿[1] 黄霖[1] 殷德振[1] 

机构地区：[1]中山大学第二附属医院骨科,广东省广州市510120 [2]中山大学第二附属医院脐血库,广东省广州市510120

出　　处：《中国临床康复》2004年第35期7986-7987,i003,共3页Chinese Journal of Clinical Rehabilitation

基　　金：国家自然科学基金(30271311);广东省自然基金(36642);广东省科技厅攻关项目(C30702);广州市科技局项目资助(2003Z3-E0011)~~

摘　　要：目的:嗅鞘细胞(olfactoryensheathingcells,OECs)植入脊髓后可促进再生的轴突长入远段脊髓,观察人胚胎嗅鞘细胞移植对成鼠脊髓轴突再生的影响。方法:实验于2003-04/2004-10在中山大学第二附属医院实验中心脐血库完成。20只清洁级雌性SD大鼠被随机分为两组,实验组10只,将分离、纯化、培养2周的人胚胎OECs,移植于大鼠半横断胸髓(T10)的两端;对照组10只同样方法给予等量的DMEM。6周后组织切片,行苏木精-伊红染色、嗜银染色以及髓鞘碱性蛋白(MBP)和抗神经生长因子受体抗体(NGFR)免疫组化检查。结果:OECs移植组6周后脊髓大体标本显示初步愈合现象;组织学观察,OECs呈NGFR阳性表达,显示OECs仍然存活,且与宿主组织整合良好;部分再生轴突长入断端间组织,呈MBP阳性表达。结论:人胚胎OECs对成鼠胸髓损伤后的轴突再生有促进作用。AIM:To observe the effect of implantation of human embryonic olfactory ensheathing cells(OECs) on transected spinal cord of adult rats based on the accelerating effect of implantation of human OECs on the growth of regenerated axons into distal segmental spinal cord. METHODS:The experiment was completed in the Cord Blood Bank of Experiment Center,the Second Affiliated Hospital of Sun Yat-sen University from April 2003 to October 2004.Twenty male rats in clean grade were divided into two groups,experiment group and control group with 10 rats in each group.OECs,separated from human embryonic olfactory bulb and purified by culture for 2 weeks,were transplanted into transected spinal cord of chest at T10 level of rats in the experiment group,while the equal Dulbecco's Modified Eagle Medium(DMEM) was injected into the rats of control group.Tissue sections were procured at 6 weeks after transplantation,and the animals were performed with hematoxylin-eosin(HE) staining,nerve silver staining and myelin basic protein(MBP) examination and nerve growth factor receptor(NGFR) immunohistochemical staining. RESULTS:The samples of injured spinal cords displayed initial healing in the experiment group 6 weeks post-transplantation morphologically.Histological observation showed that the implanted OECs still survived and integrated into the host tissues in well arrangement and the regenerative axons grew into the distal end of the imected spinal cords.Both of OECs and regenerative axons positively expressed MBP and NGFR respectively. CONCLUSION:Implantation of human embryonic OECs can promote the regeneration of axons after the spinal cord of adult rat chest is injured.

关 键 词：脊髓损伤 嗅神经 神经再生 

分 类 号：R741[医药卫生—神经病学与精神病学]