福建建瓯锥栗、茅栗遗传多样性分析  

The Genetic Diversity of Castanea heyyi and C.sequinii in Jianou by RAPD Maker

作　　者：沈永宝[1] 施季森[1] 林同龙[2] 

机构地区：[1]南京林业大学,江苏南京210037 [2]福建省南平市林业站,福建南平353001

出　　处：《南京林业大学学报（自然科学版）》2004年第6期15-17,共3页Journal of Nanjing Forestry University：Natural Sciences Edition

基　　金：国家林业局重点资助项目(2000-36)

摘　　要：利用RAPD的DNA标记技术,分析了福建建瓯锥栗和茅栗以及我国板栗栽培品种的遗传多样性,结果表明,锥栗、茅栗和板栗多态位点百分率分别为44.9%,53.7%和52.7%。遗传多样性分析表明,锥栗的有效等位基因数目(Ne)、基因多样度(h)和Shannon多样性信息指数(I)略小于茅栗和板栗,但差别不大,它们的值分别为1.6088～1.6216,0.3480～0.3529和0.5179～0.5357,显示出较高的遗传多样性。Castanea heyyi,which is one of the most important economic forest trees in China,has been cultivated for a long time in the north part of Fujian province,especially in Jianou country.However,from the second half of 1990'on,the cultivated areas in these regions have been always increased based on assarting forest sites in existence and grafting propagation.This paper studied the genetic diversity of C.heyyi,and C.sequinii in Jianou using RAPD DNA marker,and made a comparison with varieties of Chinese chestnut from all over the country.The results showed the percent polymorphic loci of C.heyyi(44.9%) was lower remarkably than that of C.sequinii(53.7%) and C.mollissima(52.7%).The further analysis on their effective allele amounts ranged from 1.608 8～1.621 6,Nei's gene diversity ranged from 0.348 0～0.352 9 and Shannon information index ranged from 0.517 9～(0.535 7),indicated higher genetic diversity in these three species.

关 键 词：遗传多样性 RAPD标记 茅栗 锥栗 板栗 

分 类 号：S722[农业科学—林木遗传育种]