转P_(SAG12-ipt)基因水稻延衰性能的初步研究  

Study on antisenescence characteristics of transgenic rice (P_(SAG12-ipt))

作　　者：王亚琴[1] 梁承邺[2] 

机构地区：[1]华南理工大学食品与生物工程学院,广东广州510640 [2]中国科学院华南植物研究所,广东广州510650

出　　处：《广西植物》2004年第6期540-543,共4页Guihaia

基　　金：广东省科技重大专项项目 ( 2 0 0 3B2 1 2 0 7)

摘　　要：研究了叶片衰老抑制基因PSAG1 2 ipt经基因枪导入籼稻不育系中A后 ,转化植株农艺性状和生理特性的变化。结果显示 ,在水稻生育期 ,转化植株相对于对照而言 ,叶绿素、蛋白质含量和SOD(超氧物歧化酶 )活性下降以及MDA(丙二醛 )相对含量上升趋势较缓 ,表现在农艺性状上是单株有效穗数、千粒重明显高于对照。说明叶片衰老抑制基因PSAG1 2 ipt确实具有延缓叶片衰老的功能 ,延长了水稻叶片利用光能的时间 ,使之积累了大量的光合产物 。The agronomic traits and physiological characteristics of transgenic plants(R 0)were studied after the leaf-specific senescence-inhibitor gene(P (SAG12-ipt))was introduced into Zhong A(cytoplasmic male sterile indica)by particle bombardment. The results showed that during the developmental stages of rice,compared with controls,the descendant trend of the contents of chlorophyll,protein and SOD activity and the upward trend of the relative content of MDA were slow,and panicles per plant and weight of 1 000-grain were high distinctly. These data indicate that ipt gene might express in the transgenic rice by the induction of SAG12 promoter, the synthesized cytokinins maybe delay the senescence process and prolong photosynthesis period of functional leaves and make them accumulate a lot of photosynthetic products and increase yields.

关 键 词：水稻 植株 叶片衰老 IPT基因 农艺性状 有效穗数 单株 对照 抑制基因 延缓 

分 类 号：Q946[生物学—植物学] S511[农业科学—作物学]