两株SIV_(mac)的滴定和特性  

The Characteristics and Titer of Strains SIV_mac

作　　者：吴小闲[1] 卢耀增[1] 魏强[1] 何伏秋[1] 秦川[1] 宋怀燕[1] 陈志伟[1] 张永容[1] 丛喆[1] 潘勇[1] 刘亚莉[1] PrestonA. 

机构地区：[1]中国医学科学院中国协和医科大学实验动物研究所 [2]Marx美国AaromDiamond艾滋病研究中心

出　　处：《中国实验动物学报》1998年第2期18-22,共5页Acta Laboratorium Animalis Scientia Sinica

摘　　要：SIVmac251的MID100为32TCID，而SIVmac239的MID100高于320TCID。体内滴定感染成功的5只猴（SIVmac2513只，SIVmac2392只）和用ZMID100SIVmac251感染的7只猴感染后有全身淋巴结肿大，并出现规律性的血浆病毒血症和抗体反应。SIVmac251感染的7只恒河猴和2只食蟹猴的淋巴结和脾脏的病理组织学检查，显现规律的SIVmac感染后的组织学变化。上述结果表明两株SIVmac均能诱发SIVmac感染猴的系列表现和变化，可应用于抗艾滋病药物猴体疗效的评价。It has estimated that MID100(100% monkey infected dose) of SIVmac251 was 32 TCID andSIVmac239 was more than 320 TCID. Three monkeys inoculated with SIVmac251, two monkeysSIVmac239 and seven monkeys SIVmac251 2 MID100 were infected pefectly. All infected monkeysappeared generalized lymphopathy, plasma viremia and antibody response regularly. The pathological examination of lymph nodes and spleens in seven Rhesus and two cynomolgus monkeysshowed infectious changes. It is quite obvious that the symptoms an sign, Pathological histologicalchanges, and viremia and antibody response of infected monkeys were caused by SIVmac251 orSIVmac239 strain. So above strains could be used to evaluate the effect of anti - AIDS drugs inmonkey test.

关 键 词：SIV 感染后 MID 淋巴结肿大 猴体 体疗 病理组织学检查 食蟹猴 滴定 抗体反应 

分 类 号：Q78[生物学—分子生物学] R512.91[医药卫生—内科学]