外源性Rb基因导入对U937细胞周期和p21基因表达的影响  

Effect of Exogenous Rb Gene on Expression of p21 and cell cycle regulation in U937 Cells

作　　者：李斌元[1] 何淑雅[1] 王桂良[1] 闾宏伟[1] 刘俊文[2] 屈顺林[2] 马云[1] 杨向东[2] 

机构地区：[1]南华大学生物化学与分子生物学教研室,湖南衡阳421001 [2]南华大学心血管病研究所,湖南衡阳421001

出　　处：《中国现代医学杂志》2004年第23期59-62,共4页China Journal of Modern Medicine

基　　金：国家自然科学基金(30200103);湖南省自然科学基金(02JJY4011)资助。

摘　　要：目的利用Rb基因重组腺病毒载体(AdCMV-Rb),将外源性Rb基因导入U937细胞,研究其对细胞周期、细胞凋亡及原癌基因p21表达的影响。方法AdCMV-Rb导入U937细胞后,用流式细胞术分析细胞周期和细胞凋亡率及Rb蛋白和p21蛋白的表达;RT-PCR检测原癌基因p21的表达。结果Ad-CMV-Rb可以高效导入U937细胞,Rb基因导入使U937细胞主要阻滞在G1期,细胞凋亡率在Rb基因导入24 h后无明显改变,48 h后凋亡率增高;Rb蛋白和p21蛋白表达增强;RT-PCR结果显示Rb基因的导入可以上调p21基因的表达。结论外源性Rb基因的导入可以上调原癌基因p21的表达,使得U937细胞周期阻滞在G1期。Objective: To study the influence of Rb gene on the cell cycle, cell apoptosis and expression of p21 in U937 cells by recombinant adenovirus vector. Methods: Cell cycle and cell apoptosis, expression of Rb and p21 protein were detected by flow cytometric analysis. Expression of p21 gene were detected by RT-PCR. Results: Rb gene could be quickly and effectively transferred into the U937cells by adenovirus vector. Cell cycle was inhibited with most of the cells arrested in G1 phase. Apoptosis rate increased remarkably after AdCMV-Rb transferred for 48 h. Rb and p21 protein expression were up-regulated. Conclusion: AdCMV-Rb transfection can inhibit the cells cycle in G1 phase by up-regulating p21, it maybe has a potential significance on atherogenesis.

关 键 词：单核细胞 视网膜母细胞瘤基因 细胞周期 P21 

分 类 号：Q786[生物学—分子生物学]