膀胱移行细胞癌中MT-3的表达及其临床意义  

Expression of metallothionein (MT-3 mRNA) in bladder tumour and its clinical significance

作　　者：朱斌[1] 杨罗艳[1] 赵晓昆[1] 

机构地区：[1]中南大学湘雅二医院泌尿外科,湖南长沙410011

出　　处：《中国现代医学杂志》2004年第23期63-65,67,共4页China Journal of Modern Medicine

摘　　要：目的探讨膀胱移行细胞癌中金属硫蛋白(MT-3 mRNA)的表达与早期诊断、临床分级和预后的相关性。方法采用逆转录多聚酶链反应(RT-PCR)技术,对12例正常膀胱黏膜和23例膀胱肿瘤MT-3 mRNA的表达进行了检测。结果12例正常膀胱黏膜中MT-3 mRNA表达为0%,18例膀胱癌MT-3 mR-NA有表达为78.3%,并对其进行了临床生物学行为的观察和比较。结论MT-3的表达与膀胱移行细胞癌的早期诊断相关,可能成为一种新的肿瘤标志物。RT-PCR方法检测膀胱肿瘤时具有相对敏感、特异和结果可靠等优点。Objective: To explore the relationship between expression of Metallothionin-3 mRNA and transitional cell carcinonma of bladder. Method: Using RT-PCR technique, expression of Metallothionin-3 mRNA (MT-3mRNA) in 12 specimen from normal bladder mucosa and 18 specimen from bladder tumor were measured. Results: The results showed that no were expressed in normal bladder mucosa, but it was that in 24 cases is 78.3%. Correlation of the MT-3 mRNA expression with the clinical biological behavior was also observed. Conclusion: MT-3 mRNA don′t exist in normal bladder mucosa, but bladder tumor has a positive MT-3 mRNA expression, but which don′t correlate with the degree of malignance. The RT-PCR assay proves to be highly senitive, relatively quantitative and reliable for measuring the level of MT-3mRNA expression in clinical specimens.

关 键 词：膀胱肿瘤 金属硫蛋白 逆转录多聚合酶链反应 

分 类 号：R392.11[医药卫生—免疫学] R737.14[医药卫生—基础医学]