口蹄疫O/China/99毒株在不同宿主系传代的3A和VP1基因变异研究  

Studies on Variations of 3A and VP1 Genes of Foot-and-mouth Disease Virus Strain O/China/99 from Serial Passages in Different Hosts

作　　者：张永光[1] 吕建亮[1] 王永录[1] 方玉珍[1] 蒋守田[1] 张维德[1] 刘湘涛[1] 潘丽[1] 刘力宽[1] 裴仉福[1] 

机构地区：[1]中国农业科学院兰州兽医研究所农业部畜禽病毒学重点开放实验室国家口蹄疫参考实验室,甘肃兰州730046

出　　处：《病毒学报》2004年第4期338-346,共9页Chinese Journal of Virology

基　　金：国家重大基础研究发展规划项目(973项目)G1999011905

摘　　要：利用RT-PCR法扩增了经不同宿主系(乳鼠、BHK21细胞、PK15细胞)连传不同代次的口蹄疫O/China/99毒株的3A和VP1基因,并进行了核苷酸和氨基酸序列分析。结果表明,该毒株经不同宿主系传至90代后,VP1基因未发生大的变异,主要抗原位点也较稳定;而非结构蛋白3A基因却在不同宿主和代次发生突变缺失;经BHK21细胞传代后未发生缺失;经PK15细胞传代,在70～90代之间在第254～313位出现缺失;经乳鼠传代,在60～70代之间在第265～300位发生缺失,并在以后的90代毒中仍在此位缺失。这与代表性猪源流行毒O/YUN/TAW/97和O/HKN/21/70的3A基因在第276～305位发生缺失有相同之处。3A and VP1 genes of foot-and-mouth disease virus strain O/China/99,which was serially passaged in different hosts(suckling mice?BHK21 cell?PK15 cell),were amplified by RT-PCR,and the sequences of nucleotides and amino acids were analyzed.The results were as follows:VP1 gene and the main antigenic sites were conserved through 90 passages in different hosts;however,the 3A gene of non-structural protein had deletions in different hosts and different passages.In detail,the 3A gene showed no deletion through BHK21 passages,but the deletions emerged between 70～90 passages at nucleotides 254～313 on PK15 cell,and between 60-70 passages at 265nt～300nt in suckling mice, deletions at nucleotides 265～300 emerged again on 90th passage of mice host,which was similar with swine-sourced prevailing virus O/YUN/TAW/97 and O/HKN/21/70 with deletions on nucleotides 276～305 of 3A gene.

关 键 词：宿主 细胞 乳鼠 VP1基因 毒株 PK 非结构蛋白3 传代 代次 口蹄疫 

分 类 号：Q813[生物学—生物工程] S858[农业科学—临床兽医学]