五株鸽副粘病毒国内分离株F基因片段的克隆与分子特性  

Molecular Characterization of F Gene Segments of Five Pigeon Paramyxovirus I Isolates Obtained from Recent Outbreaks in China

作　　者：刘华雷[1] 周斌[1] 郁斌 王永坤[3] 陈溥言[1] 

机构地区：[1]南京农业大学农业部动物疫病诊断与免疫重点开放实验室,江苏南京210095 [2]吴江市出入境检验检疫局,江苏吴江215200 [3]扬州大学畜牧兽医学院,江苏扬州225009

出　　处：《病毒学报》2004年第4期378-381,共4页Chinese Journal of Virology

摘　　要：Five isolates of pigeon paramyxovirus Ⅰ（PPMV-Ⅰ）,designated as YZ9712,NC9701,NP9904,PB9601 and SZa9803,were isolated from pigeons during epizootics recently in China.The F gene functional domains were amplified by RT-PCR and then cloned into pGEMDR○-T vector and sequenced.A phylogenetic tree based on nucleotide sequence of the F gene segment revealed all the strains were divided into seven genotypes,and five PPMV-Ⅰ isolates similar to strains 1168/84 and 760/83 were grouped into subgenotype Ⅵb.The homology between these five isolates were 98.3%～99.4% and 97.8%～99.4% at the nucleotide and amino acid levels respectively.The deduced amino acid sequence at the cleavage site of the F protein was of virulent strains112K-R-Q-K-R-F117?All the isolates had highly genetic similarity to the England reference strain 1168/84 and Wales reference strain 760/83 of PPMV-Ⅰ,suggesting that they might have originated from a common ancestor.Five isolates of pigeon paramyxovirus Ⅰ(PPMV-Ⅰ),designated as YZ9712,NC9701,NP9904,PB9601 and SZa9803,were isolated from pigeons during epizootics recently in China.The F gene functional domains were amplified by RT-PCR and then cloned into pGEM^(DR○)-T vector and sequenced.A phylogenetic tree based on nucleotide sequence of the F gene segment revealed all the strains were divided into seven genotypes,and five PPMV-Ⅰ isolates similar to strains 1168/84 and 760/83 were grouped into subgenotype Ⅵb.The homology between these five isolates were 98.3%～99.4% and 97.8%～99.4% at the nucleotide and amino acid levels respectively.The deduced amino acid sequence at the cleavage site of the F protein was of virulent strains^(112)K-R-Q-K-R-F^(117)?All the isolates had highly genetic similarity to the England reference strain 1168/84 and Wales reference strain 760/83 of PPMV-Ⅰ,suggesting that they might have originated from a common ancestor.

关 键 词：F基因 分子特性 Ⅰ型副粘病毒病 鸽瘟 接触性传染病 分离株 养鸽 克隆 RNA 节段 

分 类 号：Q422[生物学—神经生物学] S858[生物学—生理学]