三氧化二砷对雄激素非依赖性前列腺癌PC-3细胞株细胞增殖及细胞周期的影响  

Effect of arsenic trioxide mediated cell growth inhibition and cell cycle arrest in hormone refractory prostate cancer PC-3 cell line

作　　者：阳东荣[1] 陈昭典[2] 单玉喜[1] 

机构地区：[1]苏州大学附属第二医院泌尿外科,215004 [2]浙江大学医学院附属第一医院泌尿外科

出　　处：《江苏医药》2004年第12期898-900,共3页Jiangsu Medical Journal

摘　　要：目的　了解三氧化二砷对雄激素非依赖性前列腺癌PC 3细胞株细胞增殖及细胞周期的影响。方法　以不同浓度的三氧化二砷作用于PC 3细胞 ,采用四甲基偶氮唑蓝 (MTT)法、流式细胞仪、相差显微镜和透射电镜观察细胞增殖状况、细胞周期变化以及细胞形态学的变化。结果　各浓度的三氧化二砷均可抑制PC 3细胞的增殖 ,具有剂量和时间累积效应 (P <0 0 1 )。三氧化二砷可使PC 3细胞生长停滞于G1 期。G1 期细胞数目随三氧化二砷的作用浓度增高而增多。其中3μmol/L、6 μmol/L、1 0 μmol/L的三氧化二砷作用 4 8小时后 ,可见PC 3细胞凋亡 ,分别为 1 1 8%,1 2 7%,2 9 6 %。透射电镜检查 3μmol/L三氧化二砷作用 4 8小时后的PC 3细胞 ,可见明显的凋亡小体。结论　三氧化二砷可抑制PC 3细胞的增殖 ,并具有剂量和时间依赖性 ;抑制细胞增殖与细胞周期阻滞于G1 期有关 ;三氧化二砷并可诱导PC 3细胞凋亡。Objective To investigate the effect of arsenic trioxide mediated cell growth inhibition and cell cycle arrest in hormone refractory prostate cancer PC-3 cell line.Methods PC-3 cells were exposed to different concentration of arsenic trioxide with different time.The situation of cell growth inhibition,cell cycle arrest,and cell morphology were investigated by MTT method,flow cytometry,light microscopy and transmission electron microscopy.Results Arsenic trioxide inhibited PC-3 cell growth through dose and time dependent manner(P<0.01).Arsenic trioxide mediated PC-3 cell cycle arrest in G1 stage.After incubated for 72 hours,the rate of G1 cell was 44.5%,43.2%,47.4%,48.3%,55.9%,and 65.1% respectively when the concentration of arsenic trioxide was 0,1,2,3,6,and 10 μmol/L.After PC-3 cell was incubated with arsenic trioxide (3 μmol/L,6 μmol/L,10 μmol/L)for 48 hours,the cell apoptotic rates were 11.8%,12.7%,29.6% respectively(P<0.001).The apoptotic body was seen in PC-3 cells examined by transmission electron microscopy after it was treated by 3 μmol/L arsenic trioxide for 48 hours.Conclusion Arsenic trioxide inhibited PC-3 cell growth through dose and time dependent manner.This effect was related to cell cycle arrest in G1 stage.Arsenic trioxide could induce apoptosis in PC-3 cell line.

关 键 词：三氧化二砷 PC-3细胞株 细胞增殖 雄激素非依赖性前列腺癌 细胞周期阻滞 凋亡小体 抑制 时间依赖性 细胞形态学 生长 

分 类 号：R737.25[医药卫生—肿瘤] R733[医药卫生—临床医学]