HPLC测定腰痛灵栓中桂皮醛的含量及对麝香酮的鉴别  

Determination of Cinnamaldehyde and Identification of Muscone in Yaotongling Suppository by HPLC

作　　者：宋英[1] 盛蓉[1] 谈静[2] 李小年[2] 

机构地区：[1]成都中医药大学附属医院,四川成都610072 [2]成都中医药大学,四川成都610075

出　　处：《中国中医药信息杂志》2004年第12期1069-1070,共2页Chinese Journal of Information on Traditional Chinese Medicine

摘　　要：目的 建立腰痛灵栓中桂皮醛的含量测定及麝香酮鉴别的方法。方法 采用 RP-HPLC,以乙腈-0.1%磷酸(38∶62)为流动相,在检测波长 285 nm 处测定桂皮醛含量;以 2,4-二硝基苯肼为衍生试剂,乙腈-0.1%磷酸(90∶10)为流动相,在检测波长 365 nm 处鉴定麝香酮。结果 桂皮醛平均回收率为 98.75%,RSD=2.22%;样品中麝香酮衍生物得到良好分离。结论 桂皮醛 HPLC 含量测定方法可行;麝香酮 HPLC 鉴别方法可行。Objective To establish a method for the determination of cinnamaldehyde and qualitative identification of muscone in Yaotongling suppository. Methods The cinnamaldehyde was determined by RP-HPLC with a mobile phase of acetonitrile-0.1% phosphoric acid (38 ∶ 62) and the detection wavelength at 285 nm. The muscone derivative with 2,4-Dinitrophenyhydrazine was followed by RP-HPLC with a mobile phase of acetonitrile-0.1% phosphoric acid (90 ∶ 10) and the detection wavelength at 365 nm. Results The average recoveries of cinnamaldehyde was 98.75%, RSD=2.22%. This method shows good seperating degree of muscone derivative in samples. Conclusion This method can be used for the determination of cinnamaldehyde and the qualitative identification of muscone.

关 键 词：高效液相色谱法 腰痛灵栓 桂皮醛 麝香酮 

分 类 号：R285.5[医药卫生—中药学]