姜黄素对长波紫外线所致DNA损伤的拮抗作用  

Antagonistic effect of curcumin on ultraviolet A-induced DNA damage in human epidermoid carcinoma cells

作　　者：周敏[1] 周慧[2] 

机构地区：[1]太原市中心医院,030009 [2]山西省疾病预防控制中心

出　　处：《山西医药杂志》2004年第12期1031-1033,共3页Shanxi Medical Journal

摘　　要：目的　观察长波紫外线引起人表皮样癌细胞 DNA链断裂损伤的情况 ,以及姜黄素对这种损伤的拮抗作用。方法　用长波紫外线照射细胞 ,用单细胞凝胶电泳法检测 DNA损伤。结果　长波紫外线剂量依赖性地诱发表皮细胞的 DNA链断裂损伤 ,损伤的高峰出现在照射后 1h。而姜黄素可以拮抗长波紫外线引起的DNA链断裂 ,表现为彗星细胞百分比的降低和彗星尾巴长度的缩短 ,并有量效关系。结论　姜黄素可以预防长波紫外线照射引起的皮肤细胞 DNA链断裂损伤。Objective To investigate the antagonistic effect of curcumin on DNA damage in human epidermoid carcinoma cells induced by long-wavelength ultraviolet (UVA).Methods Cells were treated with UVA radiation,and DNA strand breaks were studied through using the comet assay.Results UVA radiation led to a dose-dependently increased DNA strand breakage,and the peak damage occurred at 1 h after treatment.At the same time,curcumin dose-dependently inhibited the DNA strand breaks induced by 16 kJ/m 2 UVA,as shown by the decrease of comet cells and the weakness of the comet tails.Conclusion Curcumin can prevent the DNA strand breaks induced by UVA.

关 键 词：长波紫外线 姜黄素 DNA链断裂 DNA损伤 照射 拮抗作用 表皮细胞 尾巴 彗星 量效关系 

分 类 号：R285[医药卫生—中药学]