不同生长调节剂对丹参快速繁殖的影响  

Research on Techniques of Micropropagation in vitro of Salvia miltiorrhiza Bunge

作　　者：冯玲玲[1] 周诗毅[1] 范美华[1] 周吉源[1] 

机构地区：[1]华中师范大学生命科学学院,湖北武汉430079

出　　处：《中国野生植物资源》2004年第6期54-57,共4页Chinese Wild Plant Resources

摘　　要：探讨了丹参 (SalviamiltiorrhizaBunge)快速繁殖过程中不同生长调节剂的影响。实验表明 :MS +6 BA 2 .0mg/L +NAA 0 .0 5mg/L是诱导初代培养的芽产生大量丛生芽的最佳培养基 ,其诱导生芽率为 10 0 % ;最佳的丛生芽增殖培养基为MS +6 BA 1.0mg/L +NAA 0 .0 1mg/L ,其增殖倍数为 15倍 ;MS +6 BA 0 .5～ 2 .0mg/L是诱导大量不定芽的最佳培养基 ,其诱导生芽率为 10 0 % ,最佳的不定芽增殖培养基为MS +6 BA 1.0mg/L ,其增殖倍数为 2 4倍 ;诱导生根较好的培养基为 1/ 2MS +IBA 0 .1mg/L ,生根率为 98% ,移栽成活率为 10 0 %。This paper reports the effect of different hormone on rapid propagation of Salvia miltiorrhiza Bunge.The results show that MS+6 BA 2.0 mg/L+NAA 0.05 mg/L can induce a large quantity of thicket buds with the 100% percentage of reduction,the most proper medium of bud breeding is MS+6 BA 1.0 mg/L with 15 times of breeding;MS+6 BA 0.5-2.0 mg/L is a proper medium for inducing numerous indefinite buds,the reduction percentage of which is 100%,MS+6 BA 1.0 mg/L+NAA 0.05 mg/L is the most proper medium of bud breeding with 24 times of breeding;the better medium for rooting culture is 1/2MS+IBA 0.1 mg/L,the percentage of rooting growth of which is 98%,the percentage of living plant in transplanting is 100%.

关 键 词：6-BA 培养基 生长调节剂 增殖倍数 不定芽 丛生芽 快速繁殖 MS 诱导 丹参 

分 类 号：Q945[生物学—植物学] S682[农业科学—观赏园艺]