exendin-4对链脲佐菌素造模糖尿病小鼠的药效观察  

Effectiveness of exendin-4 on blood glucose level of Streptozotocin-induced diabetic mice

作　　者：朱敏[1] 李智[2] 胡敏[2] 方京冲[2] 史虹莉[2] 

机构地区：[1]复旦大学附属华山医院老年科,上海200040 [2]复旦大学附属华山医院内分泌科,上海200040

出　　处：《上海医学》2004年第12期909-912,共4页Shanghai Medical Journal

摘　　要：目的　观察exendin- 4对链脲佐菌素 (STZ)造模糖尿病小鼠血糖的影响。方法　 8周龄的雄性C5 7BL/6种小鼠 6 0只 ,均分为 6组 ,即注射用水组、exendin 4 0 .1μg/g体重组、STZ +注射用水组、STZ +exendin 4 0 .1μg/g体重组、STZ +exendin -4 0 .2 μg/g体重组、STZ +exendin- 4 0 .4 μg/g体重组 ,记录给药期间(1～ 10d)和实验第 12、15、18、2 4天血糖及第 30天时 (观察阶段 )腹腔葡萄糖耐量试验 (IPGTT)的结果。结果　注射用水组和exendin -4 0 .1μg/g体重组给药期间及治疗后观察阶段血糖的差异无显著性(P >0 .0 5 )。STZ +不同剂量exendin 4治疗组在给药期间和治疗后观察阶段血糖较STZ +注射用水组明显降低 (P <0 .0 5 ) ;给药期间不同剂量exendin 4治疗组中 ,STZ +exendin 4 0 .1μg/g体重组的效果优于其他两组 ,其血糖值与注射用水组的差异无显著性 (P >0 .0 5 ) ;治疗后观察期间 3种治疗剂量组血糖的差异无显著性 (P >0 .0 5 )。实验 30d时 ,STZ +不同剂量exendin 4治疗组与STZ +注射用水组IPGTT结果的差异无显著性 (P >0 .0 5 )。结论　exendin- 4对正常小鼠没有致低血糖作用 ,对STZ造模的糖尿病小鼠的血糖有改善作用 ,停药后仍有一定的后续作用 ,药效与剂量不成依赖性。Objective To investigate the effectiveness of exendin-4 on glucose level of streptozotocin(STZ)-induced diabetic mice. Methods Male eight-week-old C57BL/6 mice were divided into six groups: aqua-injected group, exendin-4 0.1 μg/g weight group, STZ group, STZ+exendin-4 (0.1, 0.2, 0.4 μg/g weight) groups. The blood glucose levels were determined on the first 10 days during the treatment and on day 12, 15, 18, 24 after the treatment. All mice were subjected to an intraperitoneal glucose tolerance test (IPGTT) on day 30. Results There was no statistical difference between the groups of aqua-injected group and exendin-4 groups 0.1 μg/g weight group during and after the treatment. As compared with STZ treated group only, STZ+varying doses of exendin-4 groups showed a lower blood glucose level throughout the study (P< 0.05); the 0.1 μg/g weight dose gave the best result, there was no significant difference as compared with the aqua-injected group in normal mice (P> 0.05), and there was no notable difference among the three groups after the treatment. The results of IPGTT on 30th day showed no distinct difference either.Conclusion Exendin-4 would not lead to hypoglycemia in normal mice but can improve the hyperglycemia in STZ-induced diabetic mice, it also has a prolonged effect on blood glucose level independent of dose.

关 键 词：STZ 血糖 造模 正常小鼠 观察 糖尿病 注射用水 雄性 体重 显著性 

分 类 号：R96[医药卫生—药理学]