大鼠胸导管内皮细胞生物学特性的探讨  

Investigative the biological characters of rat thoracic duct endothelial cells

作　　者：刘宝全[1] 张雅芳[1] 李晓冬[1] 李益民[1] 赵玲辉[1] 李伟[1] 

机构地区：[1]哈尔滨医科大学解剖学教研室,黑龙江哈尔滨150086

出　　处：《哈尔滨医科大学学报》2004年第6期499-501,共3页Journal of Harbin Medical University

基　　金：国家自然科学基金资助项目 (3 0 2 70 697) ;黑龙江省自然科学基金资助项目 (D0 2 3 5 )

摘　　要：目的　通过培养大鼠胸导管内皮细胞 ,探讨其生物学特性 ,为研究淋巴管内皮细胞的功能提供体外实验材料。方法　培养的胸导管内皮细胞用常规免疫组织化学染色和半薄切片光镜观察、超薄切片电镜观察。结果　胸导管内皮细胞呈铺路石样 ,Ⅷ因子相关抗原和Flt 4 (VEGFR 3)的免疫组化染色阳性 ;光镜和电镜下具有内皮细胞的形态特征。结论　体外培养的大鼠胸导管内皮细胞具有体内淋巴管内皮细胞的生物学特征 ,可作为研究大鼠淋巴管内皮细胞功能和进一步研究癌转移机制的体外实验材料。Objective Through cultivating the rat thoracic duct endothelial cells,to investigate the biological characters,and provide the experimental materials in vitro for research on the function of lymphatic endothelial cells.Methods Immunohistochemical staining method was performed on the cultivated rat thoracic duct endothelial cells,and along with the observation both on semi-thin slice under light microscope and on ultrathin slice under TEM.Results The thoracic duct endothelial cells appeared like cobblestone,and positive results of immunohistochemical staining were gotten both on Ⅷ-related antigen and on Flt-4 (VEGFR-3),and morphology character of the endothelial cells were observed under light microscope and TEM.Conclusion The cultivated rat thoracic duct endothelial cells possess the same biological character to the lymphatic endothelial cells in vivo,and can be employed as the experimental materials in vitro in research on function of rat lymphatic endothelial cells and on the cancer metastasis mechanism.

关 键 词：大鼠 胸导管 内皮细胞 Ⅷ因子相关抗原 FLT-4 

分 类 号：R322.2[医药卫生—人体解剖和组织胚胎学]