甲醇对豚鼠单个心室肌细胞动作电位的影响  

Effects of methanol on cardiac electrophysiology isolated myocytes in guinea pig

作　　者：刘玉梅[1] 白云龙[1] 乔国芬[1] 陈辉[2] 杨宝峰[1] 

机构地区：[1]哈尔滨医科大学药理学教研室 [2]哈尔滨医科大学第二临床医学院药学部

出　　处：《哈尔滨医科大学学报》2004年第6期537-539,共3页Journal of Harbin Medical University

摘　　要：目的　研究甲醇对正常豚鼠心室肌细胞跨膜动作电位影响 ,旨在探讨甲醇对心肌细胞的电生理作用。方法　酶解法分离豚鼠单个心室肌细胞 ,应用全细胞膜片钳技术记录甲醇对豚鼠单个心室肌细胞动作电位的影响。结果　应用 0 .2 %甲醇使豚鼠单个心室肌细胞动作电位复极 5 0 %时程 (APD50 )从给药前 (12 92 .6 8± 2 98.0 3)ms缩短到 (75 0 .2 5± 6 8.0 5 )ms (n =6 ,P <0 .0 5 ) ;动作电位复极 90 %时程 (APD90 )从给药前 (132 8.13± 2 89.91)ms缩短到(783.2 5± 6 3.4 4 )ms (n =6 ,P <0 .0 5 ) ;动作电位幅度 (APA)从给药前 (12 6 .35± 3.2 0 )mV减少到 (113.2 0± 6 .0 8)mV(n =6 ,P <0 .0 5 )。结论　甲醇降低动作电位幅度 ,缩短动作电位时程 ,影响心肌正常工作 ,可能与它对心肌细胞钾通道的作用有关。Objective To investigate the effects of methanol on the action potentials in guinea pig myocardium.Methods Enzyme digestion methanol was used to isolate single ventricular myocytes and whole cell patch clamp technique was used to record action potential.Results In ventricular myocytes of guinea pig,methanol 0.2% decreased 50% repolarization of action potential duration (APD 50) from (1292.68±298.03)ms to (750.25±68.05)ms (n=6,P<0.05);90% repolarization of action potential duration (APD 90) was decreased from (1328.13±289.91)ms to (783.25±63.44)ms (n=6,P<0.05);action potential altitude(APA) decreased from (126.35±3.20)mV to (113.20±6.08)mV(n=6,P<0.05).Conclusion Methanol can decrease APA and APD by affecting the potassium currents of isolated ventricular myocytes in guinea pig.

关 键 词：甲醇 心肌细胞 膜片钳 豚鼠 

分 类 号：R331.38[医药卫生—人体生理学]