狼疮模型鼠各脏器中T细胞受体Vβ基因的表达  

Expression of T cell receptor Vβ genes in different organs of murine lupus models

作　　者：周广宇[1] 藤尾圭志[2] 俞戎[2] 山本一彦[2] 

机构地区：[1]吉林大学中日联谊医院风湿科,长春130033 [2]日本东京大学医学部风湿科

出　　处：《中华风湿病学杂志》2004年第12期733-736,共4页Chinese Journal of Rheumatology

基　　金：国家留学基金资助项目(21822097);日本文部省基金资助(平成14年12月)

摘　　要：目的探讨MRL/lpr和(NZB×NZW)F1两种狼疮模型鼠中致病性T细胞克隆在系统性红斑狼疮(SLE)发病中的作用。方法用逆转录-聚合酶链反应(RT-PCR)扩增狼疮模型鼠各脏器中的T细胞受体(TCR)Vβ基因,单链构象多态性分析法(SSCP)分析TCRVβ的表达,并应用磁珠细胞分离法(MACS)确定广泛浸润于各脏器中的T细胞的表型。结果两种狼疮模型鼠在SLE发病时,多个脏器中出现相同的T细胞寡克隆扩增带;该广泛浸润的T细胞克隆扩增呈TCRVβ限制性;此共同的T细胞克隆大多来源于CD4+T细胞。结论两种狼疮模型鼠的CD4+T细胞被活化后呈克隆扩增,广泛浸润于多脏器。Objective CD4+ T lymphocytes have been shown to play an important role in the pathogenesis of systemic lupus erythematosus (SLE). To identify the commonly accumulated T cell clones and to investigate its role in murine lupus models, it was analyzed that the T cell clonality infiltrating in different tissues derived from (NZB×NZW)F1 as well as MRL/lpr mice. Methods The expressions of T cell receptor (TCR) Vβ gene were analyzed by reverse transcriptase polymerase chain reaction (RT-PCR) combined with single-strand conformation polymorphism (SSCP) study. The phenotype of T cells expanded in different organs was determined by magnetic cell sorting (MACS). Results RT-PCR and SSCP study of TCR Vβ chain demonstrated that there were some identical T cell clonotypes expanded and accumulated in different organs in aged diseased mice. Most of these identical clonotypes were CD4+ T cells in both of the two strains. In contrast, young mice exhibited little accumulation of common clone in different organs. The TCR Vβ usage of these identical clonotypes was limited in Vβ2, Vβ6, Vβ8.1, Vβ10, Vβ16, Vβ18 in MRL/lpr mice and Vβ6, Vβ7 in (NZB×NZW)F1 mice respectively. Conclusion The results suggest that activated and clonally expanded CD4+ T cells commonly accumulated in different tissues in aged murine lupus models. These CD4+ T cell clonotypes may be involved in specific immune responses of SLE, thus playing a pathogenic role in these lupus mice.

关 键 词：狼疮 脏器 T细胞克隆 扩增 TCR 表达 T细胞受体VΒ基因 结论 模型 目的 

分 类 号：R593.241[医药卫生—内科学]