小麦T型细胞质雄性不育保持系线粒体DNA片段转化不育系的初步研究  

Study on the Fragments of mtDNA of Maintainer Line Transform T-type Cytoplasmic Male Sterility Line in Wheat

作　　者：赵宝存[1] 葛荣朝[1] 沈银柱[1] 黄占景[1] 

机构地区：[1]河北师范大学生命科学学院,河北石家庄050016

出　　处：《华北农学报》2004年第4期21-23,共3页Acta Agriculturae Boreali-Sinica

基　　金：河北省自然科学基金资助项目(303152)

摘　　要：细胞质雄性不育在植物界普遍存在,其不育机理一直是人们研究的热点。采用花粉管通道法,利用T型细胞质雄性不育保持系75 3369B线粒体的基因文库,选取其中所载外源片段较大的重组子转化T型不育系小麦75 3369A,结果重组子ME252和ME317的转化后代变成可育,表明线粒体的部分片段可以使细胞质雄性不育系的育性发生转变。Cytoplasm male sterility (CMS) exists in plant generally and it′s mechanism is the hot spot in studying. The fragments of mtDNA of maintainer line was induced into T-type wheat cytoplasmic male sterility line 75-3369A by pollen tube pathway. The fragments stem from the genome library of mtDNA of maintainer line 75-3369B. The result shows that the recomposed plasmid-ME252 and ME317 have made the fertility of 75-3369A restored. The result suggests mtDNA of maintainer might include the fertility gene.

关 键 词：细胞质雄性不育系 保持系 小麦 不育系 果重 外源 花粉管通道法 重组子 植物界 片段 

分 类 号：S511[农业科学—作物学] S565