大鼠急性心肌梗死后的心肌细胞凋亡  

Cardiomyocyte apoptosis after acute myocardial infarction in rats

作　　者：陈跃峰[1] 杨跃进[1] 陈曦[2] 阮英茆[3] 张宝杰[4] 王清峙[3] 张连庄[3] 田毅[5] 周燕文[5] 陈纪林[1] 高润霖[1] 陈在嘉[1] 

机构地区：[1]中国医学科学院中国协和医科大学心血管病研究所阜外心血管病医院冠心病诊疗中心,北京市100037 [2]中国医学科学院中国协和医科大学心血管病研究所阜外心血管病医院生化研究室,北京市100037 [3]中国医学科学院中国协和医科大学心血管病研究所阜外心血管病医院病理科,北京市100037 [4]中国医学科学院中国协和医科大学心血管病研究所阜外心血管病医院实验动物科,北京市100037 [5]中国医学科学院中国协和医科大学心血管病研究所阜外心血管病医院实验外科,北京市100037

出　　处：《中国临床康复》2004年第36期8202-8203,共2页Chinese Journal of Clinical Rehabilitation

摘　　要：目的:研究大鼠急性心肌梗死(acutemyocardialinfarction,AMI)后心肌细胞凋亡的发生情况。方法:实验选用105只雌性SD大鼠,随机抽取78只以结扎左冠状动脉前降支的方法制备AMI模型,术后24h存活的43只作为心肌梗死组;另设假手术组(n=27);各组再按观察时点随机分为48h和4周两亚组,即:心肌梗死48h(n=11)和心肌梗死4周(n=13)组,假手术48h(n=10)和假手术4周(n=10)组。末端脱氧核糖核酸转移酶介导的dUTP切口末端标记技术(TUNEL)和DNA凝胶电泳检测心肌细胞凋亡。结果:AMI大鼠梗死区心肌细胞坏死和瘢痕形成的同时,梗死/瘢痕区、梗死边缘区和非梗死区的心肌细胞凋亡指数均显著升高犤心肌梗死48h组分别为(212.86±155.75)/1000,(198.16±120.0)/1000和(63.23±45.43)/1000,心肌梗死4周组分别为(235.14±130.43)/1000,(80.33±44.29)/1000和(31.61±16.39)/1000,P<0.05～0.01犦。结论:大鼠发生AMI后,梗死区心肌细胞坏死和瘢痕形成的同时,梗死/瘢痕区、梗死边缘区和非梗死区均有心肌细胞发生凋亡。AIM:To investigate cardiomyocyte apoptosis after acute myocardial infarction(AMI) in rats.<METHODS:AMI model was established with the ligation of anterior descending branch of left coronary artery in 78 randomly selected from 105 female SD rats.Twenty four hours after operation,the 43 survivors were served as AMI group.Another 27 rats was selected randomly as sham operation group.The each group was randomly reassigned to 48 hour and 4 week subgroups according to the observation time,that was:48 hour AMI(n=11) and 4 week AMI(n=13) subgroups,48 hour sham operation(n=10) and 4 week sham operation(n=10) subgroups.Cardiomyocyte apoptosis was detected with in situ terminal deoxynucleotidyl transferase(TdT) mediated dUTP nick end labeling(TUNEL) and DNA gel electrophoresis.<RESULTS:Along with cardiomyocyte necrosis and scar formation in the infracted area in AMI rats,cardiomyocyte apoptosis index was significantly increased in the infracted/scar,border and non infarcted areas[(212.86 ± 155.75)/1 000,(198.16± 120.0)/1 000 and (63.23± 45.43)/ 1 000 in 48 hour AMI subgroup,(235.14± 130.43)/1 000,(80.33 ± 44.29)/1 000 and (31.61± 16.39)/1 000 in 24 week AMI subgroup,P< 0.05- 0.01].<CONCLUSION:After AMI in rats,cardiomyocyte apoptosis occur in the infarction/scar,border and non infarcted areas along with cardiomyocyte necrosis and scar formation in the infarcted areas.

关 键 词：心肌梗塞 细胞凋亡 大鼠 心肌/病理学 

分 类 号：R542.2[医药卫生—心血管疾病]