抗衰延寿方对小鼠染色质结构与功能损害的影响  

Effects of kangshuai yanshou compound on chromatin structure and injured function in mice

作　　者：刘群良[1] 胡梅[1] 吴琼[1] 张克纯[1] 

机构地区：[1]湖南中医学院生物化学教研室,湖南省长沙市410007

出　　处：《中国临床康复》2004年第36期8258-8259,共2页Chinese Journal of Clinical Rehabilitation

基　　金：国家中医药管理局资助课题(89212)~~

摘　　要：目的:探讨抗衰延寿方对小鼠染色质结构与功能损害的影响。方法:实验在湖南中医学院分子生物学实验室完成,选用60只小鼠,取小鼠骨髓细胞制片,镜检计数姊妹染色单体互换(SCE)、细胞分裂指数和细胞周期。结果:老年小鼠骨髓细胞SCE频率(1.79±0.35)显著高于青年小鼠(0.80±0.13),服用抗衰延寿方的老年小鼠SCE频率(1.16±0.21)显著低于对照组老年小鼠;环磷酰胺造成小鼠骨髓细胞增殖周期延长,SCE频率增加(3.73±0.57)。结论:结果表明,小鼠骨髓细胞SCE频率随增龄而增加,抗衰延寿方可阻抗小鼠骨髓细胞SCE的增龄性改变,并能有效地抑制环磷酰胺所引起的小鼠骨髓细胞分裂增殖与SCE的改变。AIM:To investigate the effects of kangshuai yanshou compound on chromatin structure and injured function in mice.<METHODS:The experiment was conducted in the Laboratory for Molecular Biology,Huan College of Traditional Chinese Medicine.The bone marrow cells were taken from 60 mice to examine frequency of sister chromatid exchange(SCE),index of cell division and cell cycles of mice under microscope.<RESULTS:The frequency of SCE in marrow cells of elder mice(1.79 ± 0.35) was significantly higher than that in marrow cells of young mice(0.80± 0.13);the frequency of SCE in marrow cells of elder mice fed with kangshuai yanshou compound(1.16± 0.21) was significantly lower than that of controls.Cyclophosphamide prolonged the proliferation cycles of the marrow cells in mice and increased the frequency of SCE(3.73± 0.57).<CONCLUSION:The frequency of SCE in mouse marrow cells increases with advance of age.The kangshuai yanshou compound can impede aging changes of the frequency of SCE in mouse marrow cells.The kangshuai yanshou compound can also effectively inhibit division and SCE changes of marrow cells in mice induced by cyclophosphamide.

关 键 词：抗衰老药(中药) 染色质 动物 实验 

分 类 号：R592[医药卫生—老年医学]