针刺对肥胖大鼠纹状体组织一氧化氮含量和一氧化氮合酶活性的作用(英文)  

Effects of acupuncture on contents of nitrogen monoxide and nitrogen monoxide synthase in the striatum of obese rats

作　　者：刘志诚[1] 孙凤岷 袁锦虹[1] 姜军作[1] 吕雅妮[1] 衣运玲[1] 

机构地区：[1]南京中医药大学第二临床医学院针灸研究所,江苏省南京市210029 [2]南京人口管理学院生殖医学教研室,江苏省南京市210042

出　　处：《中国临床康复》2004年第36期8418-8420,共3页Chinese Journal of Clinical Rehabilitation

基　　金：国家自然科学基金资助项目(39970923)~~

摘　　要：背景:已知针灸减肥机制涉及神经及神经体液调节。考虑到纹状体参与了对下丘脑摄食中枢、植物神经中枢、体温调节中枢等的调控。针刺对肥胖大鼠纹状体组织一氧化氮含量和一氧化氮合酶(nitricoxidesyn-thase,NOS)活性的调整作用如何?目的:探讨针刺对肥胖大鼠纹状体组织一氧化氮和NOS含量的影响,进一步深化针刺减肥的中枢神经作用机制。设计:以自身对照、相互对照为主的实验研究。单位:南京中医药大学第二临床医学院针灸研究所、南京人口管理学院生殖医学教研室。材料:本实验在南京中医药大学第二临床医学院针灸研究所完成。1月龄刚断乳SD雄性大鼠,体质量50～70g,由南京军区总医院实验动物中心提供,动物级别为清洁级。干预:以普通全价鼠饲料喂养的大鼠作为正常组,将造模成功的实验性肥胖大鼠随机分为对照组和针刺组,每组6只。针刺组大鼠给予针刺治疗14d,正常组和对照组大鼠分别每天放入大鼠固定器中适应15min,持续14d。采用神经生化技术观察针刺治疗前后肥胖大鼠体质量、体脂及纹状体组织一氧化氮和NOS含量的变化。主要观察指标:①针刺对实验性肥胖大鼠肥胖指标及对心包、肾周和附睾脂肪量的影响。②针刺对各组大鼠纹状体组织一氧化氮含量和一氧化氮合酶活性的影响。BACKGROUND:It is known that the mechanism of acupuncture in treating obesity comes up with the neural and neurohumoral regulations.Striatum participates in regulating and controlling food taking centrum,plant nerve centrum and animal heat centrum of hypothalamus.What is the regulating effect of acupuncture on nitric oxide (NO) content and nitric oxide synthase(NOS) activity in striatum tissues? <OBJECTIVE:To study the effects of acupuncture on the content of NO and NOS in the striatum tissue of obese rats,and further investigate the mechanism of acupuncture effect on reducing body mass in central nervous system. <DESIGN:Self controlled and mutual controlled experiment.<SETTING:Acupuncture Institute of Second Clinical Medical College,Nanjing University of Traditional Chinese Medicine,and Department of Reproduction Medicine,Nanjing College for Population Programmed Management. <PARTICIPANTS:This experiment was carried out in the Acupuncture Institute of Second Clinical Medical College.One month old male SD rats just in ablactation,weighing 50 to 70 g,were provided by the Animal Experiment Center of General Hospital of Nanjing Military Area Command ,and the animals were in the clean grade. <INTERVENTIONS:Rats fed with ordinary diet were in the normal group.Experimental obese rats models were randomly divided as control group and acupuncture group,6 in each.Rats in the acupuncture group were given acupuncture treatment for 14 days, and rats in the normal and control groups were put into rat fixation machine for 15 minutes everyday,lasting for 14 days.Body mass, body lipid and contents of NO and NOS in striatum tissues of the obese rats were observed with nervous biochemical technology before and after acupuncture respectively. <MAIN OUTCOME MEASURES:① Effect of acupuncture on obesity index,fat contents of pericardium,kidney and epididymis of obese rats.② Effect of acupuncture on NO and NOS contents in striatum tissue of obese rats.③ Correlation of NO content and NOS activity with body mass and Lee's ind

关 键 词：肥胖症/病理生理学 纹状体/针灸效应 体重 小鼠 肥胖 一氧化氮/代谢 一氧化氮合酶/代谢 针刺疗法 

分 类 号：R589.2[医药卫生—内分泌]