Phylogenetic Relationships among 12 Species of Tetrigidae (Orthoptera:Tetrigoidea) Based on Partial Sequences of 12S and 16S Ribosomal RNA  

基于线粒体12S和16SrRNA基因部分序列探讨蚱科12种的系统发育关系(英文)

作　　者：陈爱辉[1] 蒋国芳[1] 

机构地区：[1]南京师范大学生命科学学院,江苏省生物资源技术重点实验室,江苏南京210097

出　　处：《Zoological Research》2004年第6期510-514,共5页动物学研究（英文）

基　　金：国家自然科学基金资助项目 (3 0 160 0 15 )~~

摘　　要：Mitochondrial 12S and 16S ribosomal RNA genes sequences were sequenced using dye-labeled terminator on an ABI 377 automated sequencer in 11 individuals and 1 species' sequences were gained from GenBank,representing 6 genera of family Tetrigidae.The collated sequences were aligned using Clustal X version 1.81 and then,the sequence variability and heredity distances based on Kimura 2-parameter model were calculated using Mega 2.1.In obtained sequences (736 bp),the average A+T content is 73.9%,ranging from 71.2% to 77.5%;the overall G+C content is 26.1%,ranging from 22.5% to 28.8%.Based on alignment of the combined sequences,185 parsimony-informative sites were revealed in 755 available base pairs.Phylogenetic trees were reconstructed using NJ,MP and ML methods with Cylindraustralia kochii as outgroup.The results indicated that the monophyletic nature of Tetrix is questioned and suggest that T.tubercarina may be given tribal rank.Our results also show that Coptltettix huanjiangensis and C.gongshanensis are the same species,i.e.Coptltettix gongshanensis Zheng,and C.huanjiangensis is the synonyms of C.gongshanensis.用ABI 377自动测序仪测定了蚱科 5属 11个种的 12S和 16SrRNA基因部分序列 ,并从GenBank获得 1属 1种的同源序列 ;用ClustalX 1 81比较其同源性 ,用Mega 2 1计算序列变异性和遗传距离。在获得的736bp序列中 ,A +T含量为 71 2 %～ 77 5 % ,平均为 73 9% ;G +C含量为 2 2 5 %～ 2 8 8% ,平均为 2 6 1%。经ClustalX1 81软件比对 ,共得到 75 5个位点 ,其中简约信息位点 185个。以Cylindraustraliakochii为外群 ,构建NJ、MP和ML分子系统树 ,结果表明 :(1)蚱属并非一个单系群 ,而是一个并系群 ;(2 )环江柯蚱Coptltet tixhuanjiangensis和贡山柯蚱C gongshanensis为同一个种 ,即贡山柯蚱 ,而环江柯蚱是贡山柯蚱的同物异名。

关 键 词：TETRIGIDAE Phylogeny 12S rRNA gene 16S rRNA gene 

分 类 号：Q969.26[生物学—昆虫学]