后丽盲蝽属作为属级分类单元的DNA分子证据  

A DNA Molecular Evidence for Apolygus China as a Generic Taxon (Hemiptera:Heteroptera:Miridae)

作　　者：齐宝瑛[1] 郑哲民[2] 任洪宝[3] 金洪[4] 照日格图[5] 

机构地区：[1]内蒙古大学生命科学学院,内蒙古呼和浩特010020 [2]陕西师范大学生命科学学院动物研究所,陕西西安710062 [3]内蒙古师范大学生命科学与技术学院昆虫研究所,内蒙古呼和浩特010022 [4]内蒙古农业大学生态环境学院,内蒙古呼和浩特010021 [5]美国密西西比州立大学昆虫与植物病理学系,密西西比397629775

出　　处：《Zoological Research》2004年第6期515-521,共7页动物学研究（英文）

基　　金：国家自然科学基金资助项目 (3 0 460 0 2 4) ;内蒙古自治区高等学校重点项目 (2 0 0 10 2 6) ;内蒙古自治区人才开发基金(2 0 0 3 ) ;内蒙古大学生物学博士后流动站科研基金资助项目

摘　　要：用蛋白酶K消化法提取并正反链双向测得半翅目盲蝽科盲蝽族 (Mirini)中后丽盲蝽属 (Apolygus)和草盲蝽属 (Lygus) 9个种的线粒体DNA细胞色素b基因片段的序列 ,应用Mega计算该两个属 7个种 11个Cytb 4 32bp片段序列的碱基组成、核苷酸的种间变异率、所编码的 14 4个氨基酸的变异率。其核苷酸变异率 :后丽盲蝽属内小于 0 0 2 ,草盲蝽属内小于 0 0 8(个别种例外 ) ;两个属种类之间平均变异率都大于 0 10 ,平均为 0 15。氨基酸变异率 :后丽盲蝽属内种间无变异 ,而草盲蝽属内只有L gemellatus与其他种间有 13个氨基酸变异 ,仅占 9 0 3% ;两属各种类间却存在 8～ 11个氨基酸的变异。表明在分子水平上两属之间较之于属内种间具有明显的差异 ,支持将后丽盲蝽从亚属提升为属级分类单元的分类观点。Sequences of the Cyt b gene segments on mitochondrial DNA of 9 species of Apolygus China and Lygus Hahn (Heteroptera:Miridae:Mirinae:Mirini) were extracted by means of protease K digesting and sequenced to both directions.Nucleotide composition,nucleotide variation ratio between species as well as variation ratio of 144 amino acid coded by 11 segments of 433?bp of the 9 species from the two genera were calculated using phylogenetic analyzing software package Mega.Nucleotide variation ratio:less than 0.02 within Apolygus and less than 0.08 within Lygus (with the exception of a few species) but more than 0.10 between species of the two groups with the average variation of 0.15.Variation ratio of the amino acid:no difference among the species within Apolygus and Lygus themselves with only an exception of a difference of 13 amino acid between L.gemellatus and other species within Lygus,but there is stably a variation of 8 to 11 amino acids,i.e.a 5.6% to 7.6% variation between any species examined of Apolygus and that of Lygus.It is proved that there has been existed distinct difference between these two groups at molecular level and they separated very well,which strongly supports the taxonomic opinion that Apolygus is promoted to a generic taxon.

关 键 词：DNA分子 盲蝽科 属种 细胞色素B基因 碱基 半翅目 片段 变异率 氨基酸 核苷酸 

分 类 号：Q969.3576[生物学—昆虫学]