益气平悬饮对小鼠Lewis肺癌组织细胞中IL-2、TGF-β_1 mRNA表达的影响  

The Effect of Yiqipingxuan Beverage on the Expression of IL-2 and TGF-β1 mRNA in Implanting Lewis Lung Cancer among Mice

作　　者：王云启[1] 田雪飞[2] 何欣[2] 伍参荣[2] 肖雅[2] 黄立中[2] 

机构地区：[1]湖南省肿瘤医院,湖南长沙410006 [2]湖南中医学院,湖南长沙410007

出　　处：《湖南中医学院学报》2004年第6期18-20,共3页Journal of Hunan College of Traditional Chinese Medicine

基　　金：国家中医药管理局基金资助 ( 0 2 -0 3JP3 0 );湖南省自然科学基金资助 ( 0 1JJ2 0 3 7)

摘　　要：目的探讨益气平悬饮 (YQPXB)对小鼠Lewis肺癌组织细胞中白细胞介素 (IL—2 )、转化生长因子(TGF—β1)表达的影响。方法皮下接种Lewis肺癌的C57BL 6J小鼠 40只 ,随机分为益气平悬饮大剂量组、小剂量组、环磷酰胺对照组、荷瘤对照组 ,给药 1 0d后处死 ,用逆转录PCR法测定各组Lewis肺癌组织细胞中IL—2、TGF—β1mRNA表达。结果YQPXB大、小剂量组Lewis肺癌组织细胞中IL—2mRNA表达水平明显高于模型组和化疗组 (P <0 .0 1 ) ,YQPXB大、小剂量组之间无显著性差异。YQPXB大、小剂量组TGF—β1mRNA表达水平明显低于模型组和化疗组 (P <0 .0 1 ) ,YQPXB大、小剂量组之间无显著性差异。结论YQPXB能促进小鼠Lewis肺癌组织细胞中IL—2mRNA的表达 ,抑制TGF—β1mRNA的表达。Objective To investigate the effect of Yiqipingxuan Beverage (YQPXB) on the expression of interleukin-2 (IL-2) and transforming growth factor-β 1(TGF-β 1) mRNA in the tissues of implanting Lewis lung cancer among mice. Methods 40 C 57BL?/6?J mice were implanted with the cells of Lewis lung cancer by subcutaneous injection.Then, they were randomly divided into YQPXB groups at high dose and low dose, cyclophosphamide control group and tumor-bearing control group.All mice were sacrificed ten days after drug administration.The expression of IL-2 and TGF-β1 mRNA in the tissues of Lewis lung cancer of each group were determined by RT-PCR procedure. Results The expression of IL-2 mRNA was significantly higher in high and low dose of YQPXB groups than that in model group and chemotherapy group (P<0.01). There were no significant differences between high and low dose of YQPXB groups. The expression of TGF-β 1 mRNA was significantly lower in high and low dose of YQPXB groups than that in model group and chemotherapy group (P<0.01).There were no significant differences between high and low dose of YQPXB groups in this index as well. Conclusions YQPXB can enhance the expression of IL-2 mRNA and decrease the expression of TGF-β 1 mRNA in the cells of Lweis lung cancer so as to improve the expressing pattern of cytokines from Th2 to Th1.

关 键 词：益气平悬饮 LEWIS肺癌 白细胞介素-2 转化生长因子β1 小鼠 人参 生黄芪 桑白皮 

分 类 号：R285.5[医药卫生—中药学]