黑曲霉木聚糖酶同工酶的性质及圆二色谱分析  

Studies on CD spectrum properities of xylanase from Aspergillus Niger SB-02

作　　者：刘东波[1] 李丹[1] 陈珊[1] 包晓军[2] 

机构地区：[1]东北师范大学生命科学学院,吉林长春130024 [2]吉林省地震局,吉林长春130022

出　　处：《分子科学学报》2004年第4期40-43,共4页Journal of Molecular Science

基　　金：吉林省科技厅重点开发项目(990208-1)

摘　　要：　对黑曲霉木聚糖酶同工酶的酶学性质及圆二色谱(CD)进行了研究.结果表明,黑曲霉木聚糖酶粗酶液经离心、超滤及SephadexG-75凝胶层析后,分离得到了两个黑曲霉木聚糖酶同工酶组分XⅠ,XⅡ,其最适反应温度均为45℃～55℃,最佳反应pH值均为4 8～5 5,SDS-PAGE法测得XⅠ,XⅡ的相对分子质量分别为2.2×104和4.0×104.XⅠ与XⅡ的CD光谱表明,XⅠ,XⅡ分别在218和215nm处有最大负峰,说明β-折叠结构是组分XⅠ,XⅡ具有木聚糖酶活性的主要结构,也是它们发挥催化功能的结构基础.Two Xylanases XⅠ,XⅡ were purified by gel Sephadex G-100 and preparation Gel electrophoresis.The range of optimal temperatures and optimal pH of XⅠ,XⅡ for hydrolysis of xylan were 45 ℃～55 ℃ and pH=4.8～5.5 respectively.Data from the SDS-Gel electrophoresis indicated the molecular weight of XⅠ,XⅡ are 2.2×10~4 and 4.0×10~4 respectively.The analysis of CD spectrum showed that the β-pleated sheet structure of Xylanases (XⅠ),XⅡ were the main conformation with Xylanases activities.

关 键 词：黑曲霉 组分 木聚糖酶 反应温度 相对分子质量 凝胶层析 超滤 同工酶 圆二色谱 CD光谱 

分 类 号：O614.612[理学—无机化学] TQ925[理学—化学]