硝酸镧对霍霍巴多芽苗生长的促进作用及植株再生(英文)  

STIMULATION OF SHOOT PROLIFERATION BY LANTHANUM NITRATE AND PLANT REGENERATION OF JOJOBA PLANTLET IN VITRO

作　　者：徐进[1] 王玉珍[1] 罗景兰[1] 

机构地区：[1]中国科学院遗传与发育生物学研究所农业资源研究中心,石家庄050021

出　　处：《天然产物研究与开发》2004年第6期565-568,564,共5页Natural Product Research and Development

基　　金：ThisstudywassupportedinpartbythefundofCooperationprojectofinternationalsciencesandtechnologyofHebeiprovince(No.03390162D)

摘　　要：在增殖培养基 (改良MS +2mg/L6 BA +0 .5mg/LGA3 )中添加 1～ 3.5mg/L硝酸镧 ,对霍霍巴试管苗生长有显著促进作用 ,2 .5mg/L硝酸镧对多芽分化有极显著的促进 ;在生根培养基 (改良 1/2MS +3mg/LIBA +1.5mg/LNAA)中 ,1～ 2mg/L的La(NO3 ) 3 对根的分化有显著的促进作用 ,生根率提高 ,最佳浓度为 2 .0mg/L。过高的浓度对试管苗生长有一定抑制。试验表明 ,不同器官对硝酸镧的敏感程度不同。取 2cm高以上的霍霍巴试管苗 ,用 2 5mg/LIBA或NAA处理 30min ,扦插于沙基质中。保持室温 2 0～ 30℃。 5 0d后揭去覆膜 ,保持光强 30 0 0lx ,相对湿度 85 %以上。正常管理条件下成活率达 75 %。The plantlets in vitro of Jojoba were treated by 1～3.5 mg/L of Lanthanum nitrate on subculture medium(MS containing 2 mg/L 6-BA and 0.5 mg/L GA 3),the result showed a significant promotion on shoot growth and proliferation. 2.5 mg/L had a very significant effect on shoot differentiation. Roots formation could be improved by using 1～2 mg/L of Lanthanum nitrate on the rooting medium(MS 1/2 supplemented with 3 mg/L NAA and 1.5 mg/L IBA),and 2 mg/L had a very significant effect. High concentration of Lanthanum nitrate inhibited the plantlets growth. The result indicated that different plant organs had different sensitivity on the concentration of Lanthanum nitrate. 2 cm high test-tube shoots were treated by 25 mg/L IBA or NAA for 30 min,then cultivated into sand substrate. Plantlets were elaborately reared under 20～30 ℃ in a greenhouse. Uncovered the plastic membrane after 50 d. Keep the light intensity at 3000 lx and relative humidity 85% .The survival percent of the plantlets come up to 75%.

关 键 词：试管苗 芽苗生长 植株再生 硝酸镧 芽分化 生根培养基 6-BA MS 促进作用 改良 

分 类 号：Q949.751.8[生物学—植物学] S682[农业科学—观赏园艺]