成纤维细胞预处理过程及三种低温保存方法比较的实验研究  

Comparison of Three Different Methods for Cryopreservation of Fibroblast and Study on Factors Which Influence Cell Viability

作　　者：程启康[1] 杨鹏飞[1] 王欣[1] 华泽钊[1] 邓辰亮[2] 杨光辉[2] 崔磊[2] 刘伟[2] 曹谊林[2] 

机构地区：[1]上海理工大学低温生物医学技术研究所,上海200093 [2]上海市组织工程研究与开发中心,上海200235

出　　处：《制冷学报》2004年第4期20-24,共5页Journal of Refrigeration

基　　金：国家自然科学基金重点项目(59836240);973项目(G1999054309);863项目(2002AA205041)

摘　　要：成纤维细胞是构建组织工程化真皮的种子细胞,实现成纤维细胞的低温保存对成功保存组织工程化真皮有着重要的意义。采用三种不同的低温保存方法对成纤维细胞进行低温保存实验;详细介绍这三种方法的操作过程;并从细胞存活率、降温过程中的温度变化情况、操作时所需仪器设备及所耗时间等方面对这三种方法进行了比较。同时研究了DMSO的浓度、预处理时间及预处理温度等因素对成纤维细胞存活率的影响。Fibroblast is a crucial kind of cell in the construction of tissue engineered dermal equivalent. The cryopreservation of fibroblast has great significance to the tissue-engineered dermis. The pretreatment processes and three different cryopreservation methods of fibroblast were studied in this study. And the cell viability, the temperature history, and instruments required of these three methods were compared. In addition, the influences of DMSO concentration, and pretreatment time, and temperature on cell viability were also invesgated.

关 键 词：成纤维细胞 低温保存 实验研究 组织工程化真皮 种子细胞 细胞存活率 影响 预处理温度 降温 浓度 

分 类 号：TB333[一般工业技术—材料科学与工程] R619.6[医药卫生—外科学]