乙酰丙酮法测定甲壳胺寡糖数均分子量  

Determination of number average molecular mass of chitosan-oligosaccharide by acetylacetone method

作　　者：韩宝芹[1] 位晓娟[1] 房子[1] 刘万顺[1] 杨菊林[1] 

机构地区：[1]中国海洋大学海洋生命学院,山东青岛266003

出　　处：《中国海洋药物》2004年第6期12-17,共6页Chinese Journal of Marine Drugs

基　　金：国家"十五"科技攻关资助项目(2001BA708B04-07)

摘　　要：采用乙酰丙酮与氨基端反应的生色原理,将乙酰丙酮法应用于甲壳胺寡糖数均分子量的测定。通过对方法的重复性、精密性和稳定性试验,证明方法可靠。同时,通过对已报道测定甲壳胺寡糖端基相对分子质量的铁氰化钾法、HPLC法进行比较。结果表明:乙酰丙酮法比铁氰化钾法更适合甲壳胺寡糖数均分子量的测定。To determine the number average molecular mass of chitosan-oligosaccharide by u-sing the acetylacetone's method based on the color-producing reaction with amino terminal . Via the tests on the repetitiveness, accuracy and the stability, supposed this method was reliable. At the same time, the contrastive results of HPLC and the traditional K3Fe(CN)6 method indicate that the acetylacetone method was more suitable for the determination of chitosan-oligosaccharide molecular mass.

关 键 词：乙酰丙酮法 甲壳胺寡糖 数均分子量 HPLC 铁氰化钾法 

分 类 号：R917.791[医药卫生—药物分析学]