甘蓝多酚氧化酶的生物化学性质及其杀虫剂的诱导作用  

Biochemical Characteristics and Induction by Insecticides of Polyphenol Oxidase in Cabbage

作　　者：王丽红[1] 高希武[1] 

机构地区：[1]中国农业大学昆虫系,北京100094

出　　处：《农药学学报》2004年第4期33-37,共5页Chinese Journal of Pesticide Science

摘　　要：采用时间动力学法,在研究了甘蓝多酚氧化酶的最适反应条件的基础上,对有机溶剂和杀虫剂对多酚氧化酶活性的影响进行了研究。结果表明:甘蓝多酚氧化酶反应的最适pH值为6.4,最适反应温度为55℃;丙酮和乙醇对多酚氧化酶活性有明显的激活作用;高效氯氰菊酯、灭多威和辛硫磷以及小菜蛾和桃蚜取食的胁迫会对多酚氧化酶的活性产生影响。The optimum condition for determination of polyphenol oxidase(PPO) from cabbage and the induction of polyphenol oxidase by insecticides and Plutella xylostella and Myzus persicae infest were investigated. The optimum pH value and temperature are 6.4 and 55℃ for assay of polyphenol oxidase activities respectively in cabbage. Ethanol and acetone exhibit the activation to polyphenol oxidese from cabbage. α-cypermethrin, methomyl and phoxim, and the infesting of Plutella xylostella and Myzus persicae can influence the specific activity of polyphenol oxidase in cabbage.

关 键 词：多酚氧化酶 杀虫剂 甘蓝 昆虫 

分 类 号：Q946.5[生物学—植物学]