显微注射胚胎干细胞对长爪沙鼠胚胎发育的影响  

Effects of Microinjection of Embryonic Stem Cells on Development of Mouse Embryos

作　　者：周作红[1] 陈红平[1] 

机构地区：[1]江西农业大学动物科技学院,江西南昌330045

出　　处：《江西农业大学学报》2004年第6期839-842,共4页Acta Agriculturae Universitatis Jiangxiensis

摘　　要：将不同数量的成系的小鼠胚胎干细胞(ES细胞)显微注射注入长爪沙鼠(Merionesunguiculatus)的不同胚期的胚胎中,研究其对胚胎发育的影响,以期初步探索嵌合胚胎制作的条件。试验结果表明,显微注射2～5、6～10和11～15个ES细胞数量之间对早囊/囊胚期胚胎的成活率和孵化率的影响均不显著,但显微注射11～15个ES细胞的成活率和孵化率较低,分别为82.14%和64.29%;显微注射2～5个ES细胞的成活率和孵化率均较高,分别为90.90%和78.79%。显微注射11～15个ES细胞和未经显微注射的胚胎成活率分别为95.12%和82.14%,差异极显著(P<0.01);孵化率分别为90.48%和64.29%,差异极显著(P<0.01)。不经显微注射的桑椹/囊胚、经显微注射的8～16-细胞期胚胎和早囊/囊胚,其成活率分别为94.92%、91.53%和87.50%,差异均不显著(P>0.05);从孵化率来看,未经显微注射的桑椹/囊胚和经显微注射的8～16-细胞期胚胎其孵化率分别为89.83%和61.02%,差异极显著(P<0.01)。Different numbers of embryonic stem cells were injected into the different-period embryos of Meriones unguiculatus to research the effects on the embryos development and investigate the suitable conditions for making chimeric embryos. The experiment results indicated: The numbers of microinjecting ES cells(2～5,6～10,11～15), had no significant effect on both the rate of embryos development and hatch; but when microinjected with 11～15 ES cells, the rates of embryo survival and hatch were both the lowest, being 82.14% and 64.29% respectively; when microinjected with 2～5 ES cells, the rates of embryo survival and hatch were both the highest, being 90.90% and 78.79%. When microinjected with 11～15 ES cells and no microinjection, the rates of embryo survival were 95.12% and 82.14%, the difference was extremely significant(P<0.01); the rates of embryos hatch were 90.48% and 64.29%, the difference was also extremely significant(P<0.01). The blatula with no microinjection, microinjected with 8～16-cells of stage-embryos and blastula,the rates of embryo survival were 94.92%,91.53% and 87.50%, the defference was not significant(P>0.05); but with no microinjection blastula and microinjected with 8～16-cells of stage embryos, the rates of embryos hatch were 89.83% and 61.02%, the difference was extremely significant(P<0.01).

关 键 词：显微注射 胚胎干细胞 胚胎发育 

分 类 号：S861.1[农业科学—野生动物驯养]