一株腈化物降解菌的分离、鉴定及降解特性  

Screening and Identification of a Nitrile Degrading Bacterium Strain and Its Degradability

作　　者：邓林[1] 刘延岭[2] 王忠彦[1] 胡承[1] 孙勇[1] 段洪武[1] 

机构地区：[1]四川大学生命科学院,四川成都610064 [2]四川省食品发酵工业研究设计院,四川成都611130

出　　处：《精细化工》2004年第12期913-916,共4页Fine Chemicals

摘　　要：从长期被腈化物污染的土壤中分离到一株具有较宽腈化物降解利用谱的菌株YL-1。经过对YL-1形态特征及生理生化指标的分析,初步鉴定为红球菌。YL-1能降解丙烯腈生成丙烯酰胺。YL-1培养40h后可获得54U/mg的干细胞比活力。YL-1降解丙烯腈的最佳条件是温度30℃,pH=7 0,在该条件下对φ(丙烯腈)=0 2%的降解率可达99 4%。A Rhodococcus YL-1 which had high degrading capacity for nitriles was isolated and screened out from soil polluted by nitrile.YL-1 was identified morphologically and physiologically as Rhodococcus.YL-1 can catalyze and degrade acrylonitrile into acrylamide.After culturing for 40 h,54 U/mg dry cell weight of specific activity was produced.The optimal temperature and pH for YL-1 to degrade acrylonitrile were 30 ℃ and 7.0 respectively.Under above conditions,the degrading capacity of YL-1 toward φ(acrylonitrile)=0.2% is 99.4%.

关 键 词：腈化物降解 丙烯腈 红球菌 丙烯酰胺 

分 类 号：TQ085[化学工程]