抗甲胎蛋白聚苯乙烯磁珠的制备  

Preparation and Characterization of Immunomagnetic Polystyrene Bead

作　　者：邱广亮[1] 赵康[1] 李咏兰[2] 

机构地区：[1]天津大学药物科学与技术学院,天津300072 [2]内蒙古师范大学生物工程研究中心,内蒙古呼和浩特010022

出　　处：《精细化工》2004年第12期930-933,共4页Fine Chemicals

基　　金：国家自然科学基金资助项目(50066002);内蒙古自然科学基金资助项目(990303-3)

摘　　要：在磁流体存在下,进行苯乙烯-丙烯酸-马来酸酐-二乙烯基苯的共聚合,合成出粒径介于500～625nm、表面光滑、单分散的球形聚苯乙烯磁珠;磁珠耐酸碱,在浓度为1mol/L盐酸或氢氧化钠及氯化钠溶液中不凝集;控制丙烯酸用量可使磁珠表面羧基量达4 5mg/g。物理吸附法致敏磁珠的最佳条件为:包被用甲胎蛋白(AFP)抗体最适稀释度为:V(AFP抗体)∶V(磷酸氢二钠-柠檬酸缓冲液)=1∶200,最适pH=7 0,温度37℃,作用时间1h。Magnetic polystyrene beads with 500～625 nm grain size were synthesized in the presence of magnetic fluid by improved dispersion polymerization.The carboxyl groups can be impregnated on the surface of magnetic beads via copolymerization of styrene,acrylic acid,maleic anhydride and divinylbenzene.The corresponding immunomagnetic beads were prepared by adsorbing α-fetoprotein antibody onto magnetic polystrene beads for the detection of α-fetoprotein.The optimum preparation conditions were dilution of α-fetoprotein antibody=1∶200,pH=7.0,reaction temperature 37 ℃ and reaction time 1 h.The product has excellent magnetic separation and dispersion stability.

关 键 词：分散聚合 免疫磁珠 甲胎蛋白 

分 类 号：Q933[生物学—微生物学]